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Screening of Baccaurea ramiflora (Lour.) extracts for cytotoxic, analgesic, anti-inflammatory, neuropharmacological and antidiarrheal activities



It has been observed that the various part of Baccaurea ramiflora plant is used in rheumatoid arthritis, cellulitis, abscesses, constipation and injuries. This plant also has anticholinergic, hypolipidemic, hypoglycemic, antiviral, antioxidant, diuretic and cytotoxic activities. The present studyaimed to assess the cytotoxic, analgesic, anti-inflammatory, CNS depressant and antidiarrheal activities of methanol extract of Baccaurea ramiflora pulp and seeds in mice model.


The cytotoxic activity was determined by brine shrimp lethality bioassay; anti-nociceptive activity was determined by acetic acid-induced writhing, formalin- induced licking and biting, and tail immersion methods. The anti-inflammatory, CNS depressant and anti-diarrheal activities were assessed by carrageenan-induced hind paw edema, the open field and hole cross tests, and castor oil-induced diarrheal methods, respectively. The data were analyzed by one way ANOVA (analysis of variance) followed by Dunnett’s test.


In brine shrimp lethality bioassay, the LC50 values of the methanol extracts of Baccaurea ramiflora pulp and seed were 40 μg/mL and 10 μg/mL, respectively. Our investigation showed that Baccaurea ramiflora pulp and seed extracts (200 mg/kg) inhibited acetic acid induced pain 67.51 and 66.08%, respectively (p < 0.05) that was strongly comparable with that of Ibuprofen (72%) (p < 0.05). The Baccaurea ramiflora pulp and seed extracts (200 mg/kg) significantly (p < 0.05) reduced 58.5 and 53.4 in early and 80.8%, 76.61% in late phase of formalin-induced licking and biting. At 60 and 90 min pulp and seed extracts (200 mg/kg) inhibited nociception of thermal stimulus 50.16 and 62.4%, respectively (p < 0.05) which was comparable with the standard (morphine, 75.9% inhibition). The pulp and seed extracts (200 mg/kg) significantly (p < 0.05) reduced inflammation (42.00 and 55.22%, respectively) in carrageenan-induced hind paw edema and defecations (59.7 and 63.03%, respectively) in castor oil induced diarrhea. Both the extracts showed high sedative activity at 30, 60, 90, and 120 min.


Our investigation demonstrated significant cytotoxic, analgesic, anti-inflammatory, CNS depressant and antidiarrheal activities of methanol extract of Baccaurea ramiflora pulp and seeds (200 mg/kg).

Peer Review reports


Medicinal plants and natural medicines are a huge source of bioactive compounds that can be used for the discovery of new therapeutic compounds and the management of a wide range of diseases [1,2,3]. Many scientific reports showed the antidiabetic [4,5,6], anti-oxidant [7], immune stimulating [8,9,10], anti-inflammatory [11], antidiarrhoeal [7], anthelmintic [7], cytotoxic [12], and anti-obesity [13] activities of natural compounds or different herbal preparations. Baccaurea ramiflora (Lour. family of Euphorbiaceae) is a resourceful plant which has number of uses. The familiar names include Bhubi or Latkan (Bengali), Mafai (Thai), Leteku (Hindi) and Burmese grape. The slow-growing evergreen tree of Baccaurea ramiflora (B. ramiflora) has fruit (1–2″ around) and the fruit is yellow to red in color. This fruit tree is native to the Southeast Asian region and found growing wild in South China, Indo-China, India, Nepal, Myanmar, the Andaman Islands, Thailand and Peninsular Malaysia [14, 15].

The B. ramiflora is utilized as an antichloristic and anodyne against rheumatoid arthritis, abscesses, cellulitis, and treat injuries in Chinese Dai medicine [16]. The plant is also used as medicine by hill-tribes in Northern Thailand [17]. The fruit acts as antiviral and antioxidant and the stem bark acts as diuretic [18].

B. ramiflora (Lour) is such an underexploited fruit crop grown mainly in backyard plantation and as a forest plant. Research on B. ramiflora has been reported for its ethnobotanical uses, seed biology, and its isolated chemical constituents of essential oil. Three novel and four recognized compounds were isolated from the Baccaurea ramiflora stems [19]. The two new phenols, 6′- O-vanilloylisotachioside and 6′- O-vanilloyltachioside, together with nine known compounds, were isolated from the leaves of B. ramiflora (Euphorbiaceae) [17]. The rosmarinic acid that identified in Baccaurea ramiflora leaf can inhibit eicosanoids (e.g. prostaglandin biosynthesis) that is the final product of the cyclooxygenase pathway. Moreover, the phytochemical also can reduce the arachidonic acid level which indicates the antioxidant and anti-inflammatory activities of B. ramiflora [20]. The fractions of ethanol extracts of Baccaurea ramiflora (Lour.) leaves and stems showed potential cytotoxic activity [21].

B. ramiflora fruit is popular due to the high content of vitamin C, protein and iron. The plant parts are used to make wine and to treat abscesses, injuries and arthritis. They are also stewed [22]. The hydro methanol extract of the fruit pericarp of B. ramiflora showed significant DPPH scavenging activity [23]. These reviews clearly establish B. ramiflora as a medicinal plant which is underutilized and though commonly available but due to its less appealing nature and taste not gain much attention in civilized society. It tolerates unfavorable ecological condition and can be grown in unfertile lands.

These fruits have been used in folk medicine; quite a few of these are suitable for processed products. But most have not undergone any volitional stage of domestication and human selection. In animal models, phytochemical studies show various biochemical and pharmacological activities. The analysis showed that an appreciable amount of saponins and alkaloids remain in pulps (8.27 and 7.48%). The saponin containing fruits has anti-inflammatory activity [24]. The presence of alkaloids can also contribute for their analgesic, anti-apasmodic, and anti-bacterial properties [25].

Many researcher proved that flavonoids, phenolic compounds, tannins, alkaloids, saponins have analgesic, anti-inflammatory, antidiarrheal effect [25,26,27,28]. Therefore, the main objective was to assess the cytotoxic, anti-inflammatory, analgesic, CNS depressant and anti-diarrheal activities of methanol extracts of Baccaurea ramiflora pulp and seeds (MEBRP and MEBRS), respectively.


Plant material

The fresh fruits of B. ramiflora collected from the area of Rajshahi, Bangladesh. The plant was identified by a Taxonomist of Bangladesh National Herbarium, Dhaka, and a voucher specimen (38586) was retained there. Then pulp and seed were separated and dried for 1 week. Then dried plant part is pulverized into a coarse powder with a suitable grinder. The prepared powder was poured in an airtight container and placed in a cool, dark and dry place extraction.

Preparation of extracts

The pulp and seed powdered materials were placed in a fresh, smooth bottomed glass container for soaking in 85% methanol. The container was preserved up to 7 days within frequent shaking and stirring. The whole mixture was filtered through a coarse filtration material (a piece of clean and white cotton) and then filtered with Whatsman filter paper (Bibby RE200, Sterilin Ltd., UK). The filtrates (methanol extract) were evaporated using rotary evaporator and looked like a gummy concentrate black color which referred to as crude methanol extract of pulp and seed. The resulting extracts were stored in a blocked container for protection and further use.


The Swiss albino mice (male, 20-25 g) were taken from International Centre for Diarrheal Disease Research, Bangladesh (ICDDRB). Under ambient temperature all animals were kept with 12 h light followed by a 12 h dark cycle. Prior to actual experiments, the animals were acclimatized for 1 week. The animals are separated into six groups in which five mice present in each group. Experiments on animals were performed in accordance with guidelines of the Ethical Committee of Pharmacy Department, Atish Dipankar University of Science and Technology, Dhaka, Bangladesh.


Ibuprofen, diazepam and loperamide were obtained from Beximco Pharmaceuticals Ltd., Bangladesh; Merck gave formalin and acetic acid, Germany. Bangladesh. BDH chemicals Ltd. provided Tween 80, normal saline water (0.9% NaCl), castor oil, carageenan and vincristine sulphate.

Screening of cytotoxic activity

Brine shrimp lethality bioassay was determined by the method as described earlier [29]. In this suitable test simple zoological organism (Artemiasalina) was used for the screening. The brine shrimp eggs were taken from an aquarium shop (Dhaka, Bangladesh) and mature shrimp (called nauplii) hatched in artificial seawater (3.8% NaCl solution) for 48 h. The extracts was dissolved in DMSO (not more than 50 μL DMSO in 5 mL solution to avoid toxicity of itself) and sea water (3.8% NaCl in water) to prepare 10, 20, 40, 80 and 160 μg/mL concentration respectively. 50 μL DMSO was diluted to 5 mL for control group. Then mature shrimps were added to each all experimental and control vials. After 24 h the numbers of the dead nauplii were counted. After 24 h the LC50 (median lethal concentration) of the sample was calculated by a plot of percentage the dead shrimps against the logarithm of the sample concentration. Vincristine sulphate was used as a reference standard.

Determination of analgesic activity by acetic acid-induced writhing method

The acetic acid-induced writhing model was used to determine analgesic activity [30]. Test samples MEBRP, MEBRS (100 and 200 mg/kg body weight respectively), vehicle (1% tween 80 in water) and positive control (Ibuprofen, 10 mg/kg p.o.) were given and after 30 min 0.1% acetic acid was injected intra-peritoneally. The writhing (specific contractions of body) were observed randomly and its frequency was counted for up to 25 min in each group of animals [31]. Sometimes the animals showed contraction but they did not complete it which was considered as half writhing. Accordingly, two half-writhing were counted as one full writhing. The number of writhes in each sample group was compared to control group.

The percent inhibition (% analgesic activity) was calculated by

$$ \%\mathrm{inhibition}=\left\{\left(\mathrm{A}\hbox{-} \mathrm{B}\right)/\mathrm{A}\times 100\right. $$

Where, A = Average number of writhing of the control group; B = Average number of writhing of the test group.

Determination of analgesic activity by formalin test

The formalin test is used to determine the analgesic activity [31]. The MEBRP, MEBRS (100, 200 mg/kg, p.o. respectively) and Ibuprofen (10 mg/kg, p.o.) were orally administered and after 30 min 20 μL of 5% formalin was injected into the dorsal surface of the right hind paw in each group. The number of licking and biting was counted up to 30 min. The early phase time was 5 min and the late phase time was 15 to 30 min of post formalin injection. The total number of licking and biting (pain behavior) of the injured paw was calculated with a stop watch.

Determination of analgesic activity by tail immersion method

Tail immersion test was performed according to procedure as described by Olaleye SB et al. [32]. The mice tail (1 to 2 cm) was immersed in warm water kept constant at 55 ± 1 °C. The reaction time means is the time when mice deflect their tails. The first reading was discarded and the reaction time was calculated as a mean of the next three consecutive readings that was recorded at an interval of 24 h. A latency period of 28 s was distinct as complete analgesia and the evaluation was then stopped to keep away from injury of mice. The latent period of the tail-immersion response was counted at 0, 30, 60 and 90 min after the administration of standard and test drugs. Elongation percentage was calculated using the following formula

$$ \mathrm{Elongation}\%=\left\{\left(\mathrm{Latency}\ \mathrm{of}\ \mathrm{test}\ \mathrm{animal}\right)\hbox{--} \left(\mathrm{Latency}\ \mathrm{of}\ \mathrm{control}\ \mathrm{animal}\right)\right\}/\left(\mathrm{Latency}\ \mathrm{of}\ \mathrm{control}\ \mathrm{animal}\right)\times 100 $$

Determination of anti - inflammatory activity by carrageenan-induced paw oedema method

The six groups (each containing 5 mice) were taken for the test. The injected 0.1 mL carrageenan (1%) into plantar surface of mice hind paw can create acute inflammation [31]. After 30 min of carageenan injection, the treated animals received MEBRP and MEBRS (100 and 200 mg/kg, p.o), respectively. Tween 80 and Ibuprofen, (10 mg/kg, p.o.), were given in negative and positive control, respectively. The paw volume was measured at 1 h, 2 h, 3 h, and 4 h using a vernier caliper to determine the diameter of oedema.

Determination of CNS depressant activity by hole cross test

The method included a specific type of cage which consists of a steel partition that fixed in the middle of a cage having a size of 30 × 20 × 14 cm. In the center of the cage, a hole of 3 cm diameter was made at a height of 7.5 cm [33]. Animals were divided into four groups (n = 5) and each group containing four mice. Control mice received vehicle (1% Tween 80 in water), positive control received diazepam (1 mg/kg body weight, p.o.); the treated animals received MEBRP (100 and 200 mg/kg, p.o) and MEBRS (100 and 200 mg/kg, p.o), respectively. After oral administration of test drugs, the number of mice passages through the hole from one chamber to other was calculated for a period of 3 min at 0, 30, 60, 90 and 120 min.

Determination of CNS depressant activity by open field test

The experiment was carried out according to the methods described by [34]. The floor of an open field divided into alternatively colored black and white squares and the wall height was 40 cm. After giving test drugs, the number of animal movements was counted up to 3 min at 0, 30, 60, 90 and 120 min.

Determination of anti-diarrheal activity by castor oil induced diarrhea

This study was conducted by the method explained by Shoba and Thomas [35]. Initially 0.5 mL castor oil is given to each mouse for screening and only mice those showing diarrhea were chosen for the final experiment. The animals were divided into following six groups containing five mice. Control was treated with vehicle (saline 10 mL/kg, p.o.); the treated mice received MEBRP (100 and 200 mg/kg, p.o) and MEBRS (100 and 200 mg/kg, p.o), respectively. Positive control received loperamide (3 mg/kg body weight, p.o). The blotting paper was previously placed in each case and then animal was kept in an individual cage. The floor lining was changed every hour. After 30 min diarrhea was induced by oral administration of 0.5 mL castor oil. The total number of fecal output and the number of diarrheic feces excreted by the animals were recorded up to 4 h.

Statistical analysis

All values were expressed as the means ± S.E.M. of five mice (n = 5). The data were analyzed by ANOVA (Analysis of variance) followed by Dunnett’s test (Statistical Package for Social Sciences, SPSS 16.0, USA). P values < 0.05 was considered as significant.


Brine shrimp lethality bioassay

In this test the LC50 value of MEBRP 40 μg/mL, LC50 value of MEBRS 10 μg/mL where LC50 of standard (vincristine sulphate) was 0.83 μg/mL. The Brine Shrimps lethality was found to be concentration-dependent (Table 1).

Table 1 Brine Shrimp lethality bioassay of themethanolextract of the Baccaurea ramiflora pulp and seed

Analgesic activity

Acetic acid induced writhing in mice

In the acetic acid induced writhing method the MEBRP, MEBRS (200 mg/kg) showed almost same % of inhibition (67.51 and 66.08%, respectively) compared to standard (72%) and in a dose dependent manner (Fig. 1).

Fig. 1

Analgesic effects of methanol extract of the Baccaurea ramiflora pulp and seed on acetic acid-induced writhing in mice. Values are means ± SEM of five mice (n = 5); *p < 0.05 as compared to control (One way ANOVA followed by Dunnett’s test). Control mice received vehicle (1% Tween 80 in water), positive control received Ibuprofen 10 mg/kg body weight, tested animals were treated with 100 and 200 mg/kg body weight (p.o.) of the MEBRP and MEBRS, respectively

Formalin induced licking and biting test

The MEBRP, MEBRS (200 mg/kg) have shown 58.5 and 53.4% protections respectively in the early phase but in the late phase % of protections of MEBRP, MEBRS were 80.8 and 76.61% respectively where standard was 62.30 and 78.6% protection in the early and late phase respectively (Fig. 2).

Fig. 2

Analgesic effects of the methanol extract of Baccaurea ramiflora pulp and seed on hind paw licking in the formalin test in mice. Values are means ± SEM of five mice (n = 5); *p < 0.05 as compared to vehicle control (One way ANOVA followed by Dunnett’s test). Control mice received vehicle (1% Tween 80 in water); positive control received Ibuprofen 10 mg/kg body weight; tested animals were treated with 100 and 200 mg/kg body weight (p.o.) of the MEBRP and MEBRS, respectively

Tail immersion test

The maximum effect was observed at 60 and 90 min of drug administration in tail immersion test. The dose dependent 50.16%, 62.4% thermal stimulus inhibitions have shown by the MEBRP and MEBRS (200 mg/kg), respectively. In this study morphine (75.9% inhibition) was used as standard (Fig. 3).

Fig. 3

Analgesic effects of the methanol extract of the Baccaurea ramiflora pulp and seed on tail immersion test in mice. Values are means ± SEM of five mice (n = 5); * p < 0.05, Dunnett’s test compared to control. Control group received normal saline; standard groups received morphine 5 mg/kg body weight (i.p.); test animals were treated with 100 and 200 mg/kg (p.o.) body weight of the MEBRP and MEBRS, respectively

Carrageenan induced paw edema test

The MEBRP, MEBRS (200 mg/kg) exhibited moderate anti-inflammatory activity (42 and 55.22% inhibitions, respectively) and the % of inhibition of the standard (ibuprofen) was 74.62%. The anti-inflammatory activities of both pulp and seed extract were dose dependent (Table 2).

Table 2 Anti-inflammatory effects of the methanol extract of the Baccaurea ramiflora pulp and seed on carrageenan induced paw edema in mice

CNS depressant activity

Hole-cross test

MEBRP, MEBRS (200 mg/kg) showed high sedative activity at 30, 60, 90, and 120 min. Both pulp and seed have dose dependent activity and they all were statistically significant (p < 0.05) (Table 3).

Table 3 Depressant effects of the methanol extract of the Baccaurea ramiflora pulp and seed on hole cross test in mice

Open-field test

In the open field test MEBRP, MEBRS (200 mg/kg) showed same sedative activity at 60, 90, and 120 min compared to standard. The sedative effects of both pulp and seed were dose dependent (Table 4).

Table 4 Depressant effects of the methanol extract of the Baccaurea ramiflora pulp and seed on open field test in mice

Anti-diarrhoeal activity

The MEBRP, MEBRS (200 mg/kg) decreased the number of diarrhea (castor oil induced) of the test animals and the % inhibitions for defecations were 59.7 and 63.03%, respectively compared to standard (loperamide 61.34%) (Table 5).

Table 5 Antidiarrheal effects of the methanol extract of the Baccaurea ramiflora pulp and seed on castor oil induced diarrhea in mice


The diverse pharmacologic actions, cytotoxic, and pesticidal effects can be identified by the easy brine shrimp test [36]. The active plant compounds are responsible for biological responses. The brine shrimp method can determine the biological activities of natural products [37]. The B. ramiflora has brine shrimp’s mortality activity [38]. The MEBRP, MEBRS have less cytotoxic effect compared to vincristine sulphate. Moreover it is also noticeable that theLC50 value of MEBRP is higher compared toLC50 value of MEBRS. The writhing method involved peripherally acting analgesic and represents pain sensation by triggering localized inflammatory response which stimulates tissue phospholipid to release free arachidonic acid [39]. These reactions can be regulated by the prostaglandin pathways [40], peritoneal mast cells [41], and acid sensing ion channels [42].

Generally inflammatory pain is reduced by non-steroidal anti-inflammatory and analgesic drugs which can inhibit the production of pain mediators which are initiated by prostaglandins and bradykinin [43]. Therefore, the B. ramiflora might have peripheral analgesic action that influences the local reaction which is caused by the various types of production, secretion or antagonizing the action of pain mediators at the target sites. The MEBRP, MEBRS (200 mg/kg body weight) have almost same analgesic activity against pain which is caused by acetic acid in mice and these analgesic activities were very close to the reference drug (Ibuprofen). These results indicate that the B. ramiflora pulp and seed are related to the peripheral mechanisms in the analgesic action. Pritam et al. proved that compounds like flavonoids, steroids and triterpenes have anti-inflammatory and anti-nociceptive activities [44].

The formalin model is represented by neurogenic phase (0–5 min) and inflammatory pain phase (15–30 min) respectively [45]. The drugs (e.g. steroids or NSAID) primarily suppress the late phase in the CNS [46]. The neurogenic and inflammatory pains reduction by the extract might indicate the presence of analgesic compound which may work peripherally or centrally. As a result this extract can remove chronic as well as acute pain. In recent time, McNamara et al. [47] showed that formalin stimulates primary afferent neurons through a definite TRPA1 which is related to the cation channels, a subset of C-fiber nociceptors. This outcome is regulated by increased influx of Ca2+ ions. Moreover, TRPA1 cation channels mediate noxious mechanical stimuli [48]. These investigations suggested that the TRPA1 related Ca2+ mobilization is connected with mechanical stimuli and noxious chemicals since analgesic action is produced. Similarly, the inhibitory pain response of B. ramiflora might be prevent of the increase intracellular Ca2+induced by formalin. The MEBRP, MEBRS (200 mg/kg) showed significant analgesic action at both early phases and at late phase. Like acetic acid induced analgesic method formalin model also focused that both MEBRP and MEBRS (200 mg/kg) have same analgesic activity compared to standard drug and all data were statistically significant (p < 0.05).

The MEBRP, MEBRS extract (200 mg/kg, p.o.) inhibited neurogenic pain at both first and second phase induced by formalin. Thus the results indicate B. ramiflora possesses significant prostanoid mediators of inflammation [49]. The acute pain of tail immersion response is predominantly a measure for centrally acting analgesics. But in case of heat-induced pain drugs acting peripherally are inactive [50]. Latency time of mice to thermal stimuli is calculated in the tail immersion test. The different compounds in the extracts that cause calcium ions influx at the afferent nerve can also reduce adenylcyclase activity. Resulting reactions reduce cAMP level and cause an efflux of potassium ions. The resulting hyperpolarization leads to analgesic effects [51]. The tail-withdrawal time significantly (p < 0.05) increased at 30, 60 and 90 min indicate that MEBRP, MEBRS have centrally acting analgesic activity. Since the MEBRP and MEBRS (200 mg/kg) have moderate inhibition which specify the central analgesic effects of MEBRP, MEBRS. Therefore, the resulting analgesic data of three models indicate that the presence of pharmacologically active phytoconstituent (centrally and peripherally) in the MEBRP, MEBRS (200 mg/kg) extracts.

Carrageenan stimulate the release of proinflammatory and inflammatory mediators like bradykinin, leukotrienes prostaglandins, TNF-α, histamine etc. [52]. Moreover during tissue damage and inflammation neutrophils are stimulated to release excessive NO and ROS which are responsible for a variety of disease [53]. In case of experimental animal model, carrageenan is used for acute inflammation which is a biphasic. Initially, serotonin, histamine and increased synthesis of prostaglandins in the damaged tissue surroundings are involved in the first phase. Afterward, the second phase is continued by bradykinin, prostaglandins, polymorphonuclear cells, leukotrienes etc. [54, 55]. The methanol extract MEBRP, MEBRS were evaluated for in vivo analgesic and anti-inflammatory properties. Since the MEBRP, MEBRS (200 mg/kg) significantly inhibited paw edema in the second phase. This indicates inhibition of cyclooxygenase by the B. ramiflora extracts. In this test the effects were moderate compared to standard (Ibuprofen), which also inhibit the cyclooxygenase enzyme action. The pain perceptions as well as inflammations are inhibited by flavonoids and saponins which has inhibitory effects to the formation of inflammatory mediators. These results suggest that B. ramiflora may be act as an anti-inflammatory compound.

Increase of locomotor activity indicates alertness whereas decrease of locomotor activity indicates sedative effect [56]. Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter. Different drugs such as sedative-hypnotic, anxiolytic, muscle relaxant revealed their activity through GABA, therefore the methanol extracts B. ramiflora can act through enhancing GABAergic inhibition in the CNS that causes a reduction in the firing rate of critical neurons or direct activation [53] of GABA receptor. During study period of hole cross method, the MEBRP, MEBRS (100 and 200 mg/kg) have shown significant depressant activity at 30, 60, 90, 120 min. Besides this, at 30, 60, 90 and 120 min, the counted squares of each animal were also significantly decreased. In a previous study many flavonoids and neuroactive steroids may act as ligands for the GABA receptors in the central nervous system. This activity is similar to benzodiazepine like molecules [56].

The castor oil induced diarrheal effect has a number of mechanisms such as adenylatecyclase or mucosal cAMP mediated active secretion [57], inhibition of intestinal Na+, K + -ATPase activity to lessen normal fluid absorption [58], platelet activating factor and nitric oxide have contribution to the diarrheal effect [59] and prompting of prostaglandin production [60] etc.

The castor oil containing nitric oxide stimulate diarrheal activity, as well as the ricinoleic acid also created diarrhea through a hypersecretory response which is the most active chemical of castor oil [61]. In this test, the number of the feces of the test animals decreases within 4 h. The MEBRP, MEBRS (100 mg/kg) showed moderate inhibitory activity against defecation; whereas 200 mg/kg dose inhibition was close to the standard drug loperamide. Hence, these results indicate the pulp and seed extracts might have antidiarrheal action.


Our study demonstrates the effectiveness of Baccaurea ramiflora pulp and seed (200 mg/kg) extracts for analgesic, anti-inflammatory, CNS depressant and anti-diarrheal activities. Thus, the extracts are expected to contain active ingredient (s) that may contribute for the isolation of new bioactive compound (s). Hence, we suggest for further studies on the isolation and evaluation of isolated compounds in vitro and in vivo.


B. ramiflora :

Baccaurea ramiflora


Methanol extracts of Baccaurea ramiflora pulp


Methanol extracts of Baccaurea ramiflora seeds


  1. 1.

    Easmin MS, Sarker MZI, Ferdosh S, Shamsudin SH, Yunus KB, Uddin MS, Sarker MMR, Akanda MJH, Hossain MS, Khalil HA. Bioactive compounds and advanced processing technology: Phaleria macrocarpa (sheff.) Boerl, a review. J Chem Technol Biotechnol. 2015;90:981–91.

    CAS  Article  Google Scholar 

  2. 2.

    Sarker MMR, Mazumder MEH, Rashid MHO. In vitro enhancement of polyclonal IgM production by ethanolic extract of Nigella sativa L. seeds in whole spleen cells of female BALB/c mice. Bangladesh Pharm J. 2011;14:73–7.

    Google Scholar 

  3. 3.

    Goto T, Sarker MMR, Zhong M, Gohda E. Enhancement of immunoglobulin M production in B cells by the extract of red bell pepper. J Health Sci. 2010;56:304–9.

    CAS  Article  Google Scholar 

  4. 4.

    Sarker MMR, Zihad MATR, Islam M, Nahar M, Islam MH, Imam H, Ghosh A, Mustapha MH, Ismail NE. Antihyperglycemic, insulin-sensitivity and antihyperlipidemic potential of Ganoderma lucidum, a dietary mushroom, on alloxan- and glucocorticoid-induced diabetic Long-Evans rats. Func Foods Health Dis. 2015;5(12):450–66.

    Google Scholar 

  5. 5.

    Shah MA, Sarker MMR, Gousuddin M. Antidiabetic potential of Brassica OleraceaVar. Italica in type 2 diabetic Sprague Dawley (sd) rats. Int J Pharmacog Phytochem Res. 2016;8(3):462–9.

    Google Scholar 

  6. 6.

    Ullah K, Sarker MMR, Khan MS, Mustapha MS, Ullah MK. Anti-diabetic activity of compound “2-[(trimethylsilyl) oxy] - methyl ester (cas) methyl-o-trim-ethyl-silylsalicylate” isolated from Pericampylus glaucus(Lam) Merr in STZ-induced diabetic rats. J Basic ClinPharma. 2017;8:68–73.

    Google Scholar 

  7. 7.

    Karim MFB, Imam H, Sarker MMR, Uddin N, Hasan N, Paul N, Haque T. Free radical scavenging, antidiarrheal and anthelmintic activity of Pistia stratiotesL. extracts and its phytochemical analysis. Pak J Pharm Sci. 2015;28(3):915–20.

    Google Scholar 

  8. 8.

    Sarker MMR, Gohda E. Promotion of anti-keyhole limpet hemocyanin IgM and IgG antibody productions in vitro by red bell pepper extract. J Funct Foods. 2013;5:1918–26.

    Article  Google Scholar 

  9. 9.

    Sarker MM, Nahar S, Shahriar M, Seraj S, Choudhuri MS. Preliminary study of the immune stimulating activity of an ayurvedic preparation, Kanakasava, on the splenic cells of BALB/c mice in vitro. Pharm Biol. 2012;50(11):1467–72.

    Article  PubMed  Google Scholar 

  10. 10.

    Sarker MMR, Ming LC, Sarker MZI, Choudhuri MSK. Immunopotentiality of Ayurvedic polyherbal formulations “Saribadi” and “Anantamul Salsa” with augmentation of IgM production and lymphocytes proliferation: a preliminary study. Asian Pac J Trop Biomed. 2016;6(7):568–73.

    Article  Google Scholar 

  11. 11.

    Imam H, Mahbub NU, Khan MF, Hana HK, Sarker MMR. Alpha amylase enzyme inhibitory and anti-inflammatory effect of Lawsonia inermis. Pak J BiolSci. 2013;16:1796–800.

    Article  Google Scholar 

  12. 12.

    Kamaruddin MNA, Sarker MMR, Kadir HA, Ming LC. Ethnopharmacological uses, phytochemistry, biological activities, and therapeutic applications of Clinacanthus nutans (Burm. f.) Lindau: a comprehensive review. J Ethnopharmacol. 2017;206:245–66.

    Article  Google Scholar 

  13. 13.

    Kazemipoor M, Cordell GA, Sarker MMR, Radzi CJWM, Hajifaraji M, Kiat PE. Alternative treatments for weight loss: safety/risks and effectiveness of anti-obesity medicinal plants. Int J Food Prop. 2015;18:1942–63.

    Article  Google Scholar 

  14. 14.

    Khan B. Encyclopedia of Flora and Fauna of Bangladesh. Asia Soci of Bang Dhaka. 2008;1(7):392–3.

    Google Scholar 

  15. 15.

    Hoang SV, Pieter B, Keler PJA. Uses and conservation of plant species in a national park- a case study of Ben En, Vietnam. Eco Botany. 2008;62(4):574–93.

    Article  Google Scholar 

  16. 16.

    Lin YF, Yi Z, Zhao YH. Chinese Dai medicine colorful illustrations. 1st ed. Yunnan: Nationality Press; 2003.

    Google Scholar 

  17. 17.

    Yang XW, Wang JS, Ma YL, Xiao HT, Zuo YQ, Lin H, He HP, Li L, Hao XJ. Bioactive phenols from the leaves of Baccaurea ramiflora. Planta Med. 2007;73:1415–7.

    CAS  Article  PubMed  Google Scholar 

  18. 18.

    Aswal BS, Goel AK, Kulshrestha DK, Mehrotra BN, Patnaik GK. Screening of Indian plants for biological activity: part XV. Indian J Exp Biol. 1996;34:444–67.

    CAS  PubMed  Google Scholar 

  19. 19.

    Ali MR, Hossain M, Runa JF, Hasanuzzaman M. Preliminary cytotoxic activity of different extracts of Averrhoa bilimbi (fruits). Intern Curr Pharm J. 2013;2(3):83–8.

    Article  Google Scholar 

  20. 20.

    Talambedu U, Sushil KM, Malay B, Prakash L, Arvind KG. Rosmarinic acid, a new polyphenol from Baccaurea ramiflora Lour. Leaf: a probable compound for its anti-inflammatory activity. Antioxidants (Basel). 2014;3(4):830–42.

    Article  Google Scholar 

  21. 21.

    Rahman MAA, Moon SS. Antioxidant polyphenol glycosides from the plant Draba nemorosa. Bull Kor Chem Soci. 2007;28(5):827–31.

    CAS  Article  Google Scholar 

  22. 22.

    Pan ZH, Ning DS, Huang SS, Wu YF, Luo TDL. A new picrotoxane sesquiterpene from the berries of Baccaurea ramiflora with antifungal activity against Colletotrichum gloeosporioides. Nat Prod Res. 2015;29(14):1323–7.

    CAS  Article  PubMed  Google Scholar 

  23. 23.

    Jing X, Hua-shi G, Qiang L. A new sesquiterpene lactone from root of Baccaurearamiflora. Chin Trad Herb Drugs. 2007;10:1450.

    Google Scholar 

  24. 24.

    Sonia M, Gouri S, Rajinder KG. Evaluation of nutritional and phytochemical profiling of Baccaurea ramiflora Lour.syn. Baccaurea sapida (Roxb.) Mull. Arg. Fruits. Ind J Trad Know. 2016;15(1):35–14.

    Google Scholar 

  25. 25.

    Okwu DE, Okwu ME. Chemical composition of Spondiasmombinlinn plant parts. J Sustain Agric. 2004;6:140–7.

    Google Scholar 

  26. 26.

    Luis GDS, Balangood TD, Abucay JJB, Wong FM, Balangood KD, Ajifi NIG, Apostol OG. Phytochemical and antimicrobial screening of indigenous species that have potential for revegetation of landslides in Atok, Benguet, Philipines. Indian J Tradit Knowl. 2014;13(1):56–62.

    Google Scholar 

  27. 27.

    Hossein ZH, Younesi HM. Antinociceptive and anti-inflammatory effects of Crocus sativus L. stigma and petal extracts in mice. BMC Pharmacol. 2002;2:7–16.

    Google Scholar 

  28. 28.

    Viana GSB, Bandeira MAM, Moura LC, Souza-Filho MVP, Matos FJA, Ribeiro RA. Analgesic and anti-inflammatory effects of the tannin fraction from Myracrodruon urundeuva Fr. All. Phytother Res. 1998;11:118–22.

    Article  Google Scholar 

  29. 29.

    Pelzer LE, Guardia T, Juarez AO, Guerreiro E. Acute and chronic anti-inflammatory effects of plant flavonoids. IL Farmaco. 1998;53:421–42.

    CAS  Article  PubMed  Google Scholar 

  30. 30.

    Galvez J, Zarzuelo A, Crespo ME, Lorento MD, Ocete MA, Jimenez J. Antidiarrhoeic activity of Euphorbia hirta extract and isolation of an active flavonoid constituent. Planta Med. 1993;59:333–6.

    CAS  Article  PubMed  Google Scholar 

  31. 31.

    Loganga OA, Vercruysse A, Foriers A. Contribution to the ethanobotanical, Phytochemical and pharmacology studies of traditionally used medicinal plant in the treatment of dysentery and diarrhoeal in Lomelaarea, Democratic Republic of Congo (DRC). J Ethnopharmacol. 2000;71:411–23.

    Article  Google Scholar 

  32. 32.

    Zimmermann M. Ethical guidelines for investigations of experimental pain in conscious animals. Pain. 1983;16:109.

    CAS  Article  PubMed  Google Scholar 

  33. 33.

    Meyer BN, Ferrigni NR, Putnam JE, Jacobsen JB, Nicholsand DE, Mclaughlin JL. Brine shrimp; a convenient general bioassay for active plant constituents. Planta Med. 1982;45:31–4.

    CAS  Article  Google Scholar 

  34. 34.

    Zhao GX, Hui Y, Rupprecht JK, McLaughlin JL, Wood KV. Additional bioactive compounds and trilobacin, a novel highly cytotoxic acetogenin, from the bark of Asimina triloba. J Natu Prod. 1992;55:347–56.

    CAS  Article  Google Scholar 

  35. 35.

    Winter CA, Risley EA, Nuss GW. Carrageenan induced oedema in hind paws of the rats as an assay for anti-inflammatory drugs. Proc Soc Exp Biol Med. 1962;111:544–57.

    CAS  Article  PubMed  Google Scholar 

  36. 36.

    Achinta S, Mohammad AM, Sitesh CB, Joydeb KK, Bidyut KD, Lutfun N, Satyajit DS. The analgesic and anti-inflammatory activities of the extracts of Phyllanthus reticulatus in mice model. Pharm Biol. 2007;45(5):355–9.

    Article  Google Scholar 

  37. 37.

    Olaleye SB, Farombi EO, Adewoye EA, Owoyele BV, Onasanwo SA, Elegbe RA. Analgesic and anti-inflammatory effects of Kolaviron (a garcinia kola seed extract). Afr J Biomed Res. 2000;3:171–4.

    Google Scholar 

  38. 38.

    Takagi K, Watanabe M, Saito H. Studies on the spontaneous movement of animals by the hole cross test: effect of 2-dimethylaminoethane. Its acylates on the central nervous system. Jpn J Pharmacol. 1971;21:797.

    CAS  Article  PubMed  Google Scholar 

  39. 39.

    Gupta BD, Dandiya PC, Gupta ML. A psychopharmacological analysis of behaviour in rats. Jpn J Pharmacol. 1971;21(3):293–8.

    CAS  Article  PubMed  Google Scholar 

  40. 40.

    Shoba FG, Thomas M. Study of antidiarrheal activity of four medicinal plants in castor oil induced diarrhea. J Ethnopharmacol. 2001;76:73-76.

  41. 41.

    MacLaughin JL, Chang CJ, Smith DL. “Bench-top” bioassays for the discovery of bioactive natural product: an update. In: Rahman AU, editor. Studies in natural product chemistry, vol. 9. Amster: Elsevier Sci Publi BV; 1991. p. 383–409.

    Google Scholar 

  42. 42.

    Chanda S, Baravalia Y. Brine shrimp cytotoxicity of Caesalpinia pulcherrima aerial parts, antimicrobial activity and characterization of isolated active fractions. Nat Prod Res. 2011;25:20.

    Article  Google Scholar 

  43. 43.

    Raghavendra HL, TRP K, Valleesha NC, Sudharshan SJ, Chinmaya A. Screening for cytotoxic activity of methanol extract of putranjiva roxburghii wall (Euphorbiaceae) seeds. Pharmacog J. 2010;2(10):335–7.

    CAS  Article  Google Scholar 

  44. 44.

    Ribeiro RA, Mariana LV, Sara MT, Adriana BPP, Steve P, Sergio HF. Involvement of resident macrophages and mast cells in the writhing nociceptive response induced by zymosan and acetic acid in mice. Eur J Pharmacol. 2000;387:111–8.

    CAS  Article  PubMed  Google Scholar 

  45. 45.

    Kim HP, Son KH, Chang HW, Kang SS. Anti-inflammatory plant flavonoids and cellular action mechanism. J Pharm Sci. 2004;96:229–45.

    CAS  Article  Google Scholar 

  46. 46.

    Voilley N. Acid-sensing ion channels (ASICs): new targets for the analgesic effects of Non-Steroid Anti-Inflammatory Drugs (NSAIDs). Curr Drug Targets Inflamm Allergy. 2004;3:71–9.

    CAS  Article  PubMed  Google Scholar 

  47. 47.

    Nicolas V, Jan DW, Julien M, Michel L. Nonsteroidanti-inflammatory drugsinhibit both the activity and the inflammation-induced expression of acid-sensing ion channels in nociceptors. J Neurosci. 2001;21(20):8026–33.

    Google Scholar 

  48. 48.

    Pritam SJ, Amol TA, Shanjoy BB, Shanjoy JS. Analgesic activity of Abelmoschus monihot extracts. Int J Pharmacol. 2011;7:716–20.

    CAS  Article  Google Scholar 

  49. 49.

    Hunskaar S, Hole K. The formalin test in mice: dissociation between inflammatory and non-inflammatory pain. Pain. 1987;30:103–14.

    CAS  Article  PubMed  Google Scholar 

  50. 50.

    Alam B, Sarowar H, Razibul H, Anwarul I. Antioxidant and analgesic activities of Lannea coromandelica Linn. bark extract. Int J Pharmacol. 2012;8:224–33.

    Article  Google Scholar 

  51. 51.

    McNamara CR, Mandel BJ, Bautista DM, Siemens J, Deranian KL, Zhao M, Hayward NJ, Chong JA, Julius D, Moran MM, Fanger CM. TRPA1 mediates formalin-induced pain. Proc Nat Aca Sci USA. 2007;104:13525–30.

    CAS  Article  Google Scholar 

  52. 52.

    Kerstein PC, Camino DD, Morgan MM, Stucky CL. Pharmacological blockade of TRPA1 inhibits mechanical firing in nociceptors. Mol Pain. 2009;5:19–25.

    Article  PubMed  PubMed Central  Google Scholar 

  53. 53.

    Sawatzky DA, Megson IL, Rossi AG. Sildenafil offers protectionagainst NSAID-induced gastric injury. Br J Pharmacol. 2005;146:477–8.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  54. 54.

    Srinivasan K, Muruganandan S, Lal J, Chandra S, Tandan SK, Raviprakash V, Kumar D. Antinociceptive and antipyretic activities of Pongamia pinnata leaves. Phytother Res. 2003;17:259–64.

    CAS  Article  PubMed  Google Scholar 

  55. 55.

    Yaksh TL. Spinal systems and pain processing: development of novel analgesic drugs with mechanistically defined models. Trends Pharmacol Sci. 1999;20:329–37.

    CAS  Article  PubMed  Google Scholar 

  56. 56.

    Crunkhorn P, Meacock SC. Mediators of the inflammation induced in the rat paw by carrageenin. Br J Pharmacol. 1971;42:392.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  57. 57.

    Bhandare AM, Kshirsagar AD, Vyawahare NS, Hadambar AA, Thorve VS. Potential analgesic, anti-inflammatory and antioxidant activities of hydroalcoholic extract of Areca catechu L. nut. Food ChemToxicol. 2010;48:3412–7.

    CAS  Article  Google Scholar 

  58. 58.

    Antonio AM, Brito ARMS. Oral anti-inflammatory and anti-ulcerogenic activities of a hydroalcoholic extract and partitioned fractions of Turnera ulmifolia (Turneraceae). J Ethnopharmacol. 1998;61:215–28.

    CAS  Article  PubMed  Google Scholar 

  59. 59.

    Gupta M, Mazumder UK, Gomathi P, Selvan VT. Anti-inflammatory evaluation of leaves of Plumeria acuminate. BMC Com Alt Med. 2006;6:36–9.

    CAS  Article  Google Scholar 

  60. 60.

    Hossain MM, Biva IJ, Jahangir R, Vhuiyan MMI. Central nervous system depressant and analgesic activity of Aphanamixis polystachya (Wall.) parker leaf extract in mice. Afr J Pharm Pharmacol. 2009;3:282–6.

    Google Scholar 

  61. 61.

    Nell G, Rummel W. Action mechanism of secretagogue drugs. In: Csaky TZ. Pharmacol of Intestinal Permeation II, Handbook of Exp Pharmacol. 1984;70(2):461-508.

    Google Scholar 

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The authors are thankful to the Atish Dipankar University of science and Technology, Bangladesh for providing facilities to conduct this study.


The authors have not received any fund to conduct this study.

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MLN conceived, designed the research and drafted the manuscript; SMSK and KA performed experiments. MMRS critically revised the manuscript including the drawing of the figures and tables and statistical analysis, solved technical issues including data analysis and interpretation of results and assisted to resolve the queries of the reviewers. MKS, KN performed experiments; AK and MMI participated in designing the study and reviewed the manuscript. MA and MSM assisted the research work. All authors have read and approved the final manuscript.

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Correspondence to Mst. Luthfun Nesa.

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Nesa, M.L., Karim, S.M.S., Api, K. et al. Screening of Baccaurea ramiflora (Lour.) extracts for cytotoxic, analgesic, anti-inflammatory, neuropharmacological and antidiarrheal activities. BMC Complement Altern Med 18, 35 (2018).

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  • Baccaurea ramiflora
  • Cytotoxicity
  • Analgesic
  • Anti-inflammatory
  • CNS depressant
  • Antidiarrheal