Skip to main content

Pharmacological profile of the aerial parts of Rubus ulmifolius Schott



As aerial parts of Rubus ulmifolius contains phytochemicals like flavonoids and tannins. And whereas flavonoids and tannins have antioxidant and antipyretic activity, hence, current work is carried out to screen crude methanolic extract of aerial parts of Rubus ulmifolius (Ru.Cr) and crude flavonoids rich extract of Rubus ulmifolius (Ru.F) for possible antioxidant and antipyretic activity. Ru.Cr and Ru.F are also tested for brine shrimps lethality bioassay. Ru.F is tested for the first time for possible antioxidant and antipyretic activity.


Preliminary phytochemical screening of Ru.Cr and Ru.F was performed as it provides rapid finger printing for targeting a pharmacological activity. Acute toxicity and Brine shrimps’ cytotoxicity studies of Ru.Cr and Ru.F were performed to determine its safe dose range. Antioxidant and antipyretic studies were also performed as per reported procedures.


Ru.Cr tested positive for presence of tannins, alkaloids, flavonoids and steroids. Ru.Cr is safe up to 6 g/kg following oral doses for acute toxicity study. Ru.Cr is safe up to 75 μg/kg (p.o), LC50 for Ru.Cr and Ru.F are 16.7 ± 1.4 μg/ml 10.6 ± 1.8 μg/ml, respectively (n = 3). Both Ru.Cr and Ru.F demonstrated comparable antioxidant activity using vitamin C as standard (p ≤ 0.05). In test dose of 300 mg of Ru.Cr, rectal temperature was reduced by 74% (p ≤ 0.05) on 4th hour of the administration. More, Ru.F produced 72% reduction in pyrexia (p ≤ 0.05) on 4th hour of administration of paracetamol in Westar rats.


The current work confirms that aerial parts of Rubus ulmifolius contain flavonoids that are safe up to 6 g/kg (p.o). Crude methanolic extract and flavonoids rich fraction of Rubus ulmifolius have significant antioxidant and antipyretic activity. Further work is required to isolate the pharmacologically active substances for relatively safe and effective antipyretics and antioxidants.

Peer Review reports


Most of the people in world uses medicinal plants for treatment of different ailments as medicinal plants are rich source of drugs from natural sources [1]. The genus Rubus consists of more than 750 species and 12 subgenera. The genus is distributed all over the world, except Antarctica [2, 3]. Uses of Rubus species in folk medicine is based on its ethno-medicinal uses [4]. Different species of genius Rubus synthesize several varieties of medically active phytochemicals. Most of the phytochemicals are derivatives of phenolics, alkaloids, glycosides and terpenoids. Phenolics are most active biochemical constituents in species of Rubus [3]. Phenolics of Rubus include potent in vitro antioxidants, flavonoids, including compounds like flavanones, isoflavones, flavones, anthocyanins and catechins [5, 6]. Paste of roots of Rubus ellipticus is used to relieve severe headache [7]. In folk medicine, Rubus idaeus is used for treatment of colic pain and wounds in many countries. Extract of fruits of wild raspberry is used as diuretic [8]. Fruits of other species like Rubus coreanus is aphrodisiac, anti-inflammatory [9], anti-bacterial [10], antioxidant [11], anti-allergic [12] and anticancer [13] in traditional medicine. Traditionally, Rubus fruticosus has been in use for treatment of skin diseases, itching, scabies and eczema [14].

Rubus ulmifolius belongs to family Rosaceae [14]. In Italian traditional medicines, Rubus ulmifolius, has been in use for treatment of ulcers, abscesses, furuncles, red eyes, vaginal disorders, intestinal inflammations, diarrhea and hemorrhoids [15]. It is also used as antipyretic and carminative agent [16]. In Chilean folk medicine, Rubus ulmifolius is used for hypoglycemic activity [17]. It contains organic acids, ascorbic acid, volatile oils and tannins [18]. More, it has been reported that Rubus ulmifolius contains phenolics and flavonoids. Its key compounds are caffeic acid, ferulic acid, quercetin-3-O-glucuronide, kaempferol-3-O-β-d-glucuronide, gallic acid, ferulic acid and tiliroside [19]. Investigations for safe and effective herbal remedies for potent antipyretic activity gained momentum in recent as aspirin, paracetamol and nimesulide are toxic [20]. Thus safe and effective antipyretics are the need of time, preferably from plants’ kingdom. Flavonoids are found in different types of vegetables and fruits. Nuts, seeds, stems, flowers, tea, wine and honey are rich sources of flavonoids. Common constituents of chief dietary sources are flavonols, flavones, isoflavones, flavonones and biflavones [2123]. Daily flavonoids intake ranges in 1–2 g/day [24]. On the other hand, flavonols and flavones daily intake was found as 23 mg, amongst which flavonol quercetin is 16 mg/day [25]. This represents that flavonoids, in general, are very safe. Flavonoids that demonstrate well defined structure activity relationships include flavones, flavanones, flavanonols, flavans, flavonols, catechins, anthocyanidins and isoflavone. Biological activities of the major flavonoids include anti-inflammatory, antioxidant, anticancer, antiviral and antibacterial. It has been reported that flavonoids have direct cyto-protective effect on coronary and vascular systems, the liver, and the pancreas. These distinctive characteristics of flavonoids place them as preferred phytochemicals amongst the natural products [26]. Rutin, quercetin (flavonols) and hesperidin (flavanone) have inhibited inflammation. Rutin is used in treatment of chronic phase of inflammatory arthritis [27]. Similarly, quercetin has been effective in reducing rat paw edema induced by carrageenan [28]. More, flavonoids have inhibited the enzymatic activity of cyclooxygenases and lipoxygenases that ultimately help as anti-inflammatory and antipyretic [29, 30] activity with decrease in inflammatory cytokines [31]. Flavonoids are very potent antioxidants against free radicals as well. They are described as free-radical scavengers like rutin and apigenin [3235]. Free radical scavenging capacity is primarily accredited to high reactiveness of their hydroxyl substituents that take part in the reaction [25]. Due to their variety of pharmacological activities, flavonoids are referred as “nutraceuticals” [33, 3638]. As Rubus ulmifolius is rich with flavonoids and phenolics that have antioxidant effects. In addition, rutin and are flavonoids that have been reported to have anti-inflammatory effects by preventing prostaglandins synthesis, hence, in the present investigations, an attempt has been made to evaluate pharmacological activities of aerial parts of Rubus ulmifolius on the basis of the reported phytochemical constituents like flavonoids. More, there are no reports for pharmacological screenings of total flavonoids of Rubus ulmifolius. Because of the potential antioxidant and antipyretic activity of flavonoids, we targeted Rubus ulmifolius for possible antipyretic activity. Acute toxicity and brine shrimp cytotoxicity of the extract as well as of the flavonoids are also performed to determine its safe dose range.


Plant materials

Fresh aerial parts of Rubus ulmifolius were collected in month of August 2012 from Chakdara (Dir Lower), Khyber Pakhtunkhwa Province of Pakistan. The plant was identified by Dr. Nasr Ullah Khan. A voucher specimen RU/023/013 was submitted to the herbarium of University of Malakand Chakdara, Dir Lower, Khyber Pakhtunkhwa.

Preparation of crude extract

The collected aerial parts of Rubus ulmifolius were properly cleaned and subjected to shade drying. The dried plant materials (6.50 Kg) were mashed to fine powder using a mechanical grinder. The grinded materials were soaked in 11 liters of methanol (commercial grade) for 15 days. The soaked plant materials were frequently shaken and then filtered through Whatman Filter paper. The process was repeated three times. The filtrates were combined and concentrated using a rotary evaporator till a thick gummy and dark greenish crude extract (285.0 g) of Rubus ulmifolius (Ru.Cr) was obtained.

Preparation of crude flavonoids rich extract

For extraction of crude flavonoids rich extract, shade dried materials of aerial parts of Rubus ulmifolius (180 g) were finely powdered through mesh size number 40. The crushed material was filled in thimbles and was made defatted through uninterrupted hot extraction by n-hexane. The menstruum was successively extracted with methanol using Soxhlet apparatus for 12 h till darkish green mass was obtained. The contents were concentrated through evaporation. It yielded 27 g of crude flavonoids rich extract Rubus ulmifolius (Ru.F).


Albino mice (either sex) weighing in range 25–30 g were used in acute toxicity screening. Westar rats with average weight 180–200 g (either sex) were obtained from the animal house of University of Malakand, Khyber Pakhtunkhawa, Pakistan. Rats were used for screening of antipyretic activity. The animals were treated as per “Byelaws of the Scientific Procedures Issue-1 (2008) of University of Malakand”. The animals were kept in dry and clean cages on temperature 25 ± 3°C with 12h:12h dark/light cycle; relative humidity of 45–55%. The animals for the experiments were fasted overnight. However, they had free access to water. Ethical Committee of Department of Pharmacy, University of Malakand accorded approval as per “Byelaws of the Scientific Procedures Issue-1 (2008) of University of Malakand”.


All data are expressed as mean ± standard error of mean (SEM). Student’s t-test is applied using Graph Pad Prism with p < 0.05.

Pharmacological activities

Preliminary phytochemical screening

Ru.Cr was exposed to preliminary phytochemical examination for detection of terpenoids, flavonoids, glycosides, tannins, saponins, alkaloids, phenolic compounds, proteins, sterols and carbohydrates [3941]. Similarly, Ru.F was also tested for flavonoids.

Acute toxicity studies

Either sex Albino mice (weighing 25–30 g) were used for the evaluation of acute toxicity studies of Ru.Cr and Ru.F. Acute toxicity studies were conducted in two phases. Mice were fasted overnight. In phase 1, animals were allocated into three groups each having 3 mice. Ru.Cr was administered in test doses in 10, 100 and 1000 mg/kg. The animals were constantly observed for any behavioral alteration or death toll with follow up for 2 weeks. In 2nd phase, additional three groups, all having 3 mice in each, received doses of 2000, 4000 and 6000 mg/kg of Ru.Cr per oral route, respectively. All animals were continuously observed for period of 2 weeks for behavioral changes and death toll.

Same procedure was used to determine the acute toxicity studies of Ru.F. Changes in behavior and death toll were recorded [42].

Brine shrimp cytotoxicity study

Brine shrimp lethality bioassay was performed for assessment of cytotoxic activity of Ru.Cr and Ru.F. A plastic container which contained filtered artificial sea water already prepared by the dissolving 38.0 g of sea salt in 1 liter of distilled water was used in the experiments. Its pH was maintained on 8.5 that promotes the hatching of Brine shrimp (Artemia salina) eggs [43]. The container was incubated on room temperature for the next 48 h. After the hatching of eggs, live (larvae) nauplii which had got separated from the shells were obtained from the lightened part of compartment. Then, stock solutions of 10 mg/ml of both crude methanolic and the crude flavonoids extracts of Rubus ulmifolius were prepared by dissolving 50 mg of both the extracts (samples) in 5 ml distilled methanol each. From the stock solutions, different dilutions i.e. 2.5, 5, 10, 12.5, 25, 50, 75, 100, 250 and 500 μl were prepared in separate vials and left overnight to evaporate the solvent. Each vial (after solvent evaporation) was hosted with ten nauplii (10 brine shrimp larvae) through micro pipette. Volume in every vial was made with sea water up to 5 ml. All vials were maintained for 24 h at room temperature. Numbers of dead and survived shrimps were counted. The experiments were run in triplicate.

Antioxidant activity

The antioxidant aspect of Ru.Cr and Ru.F were determined through % scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical [44]. Ascorbic acid was dissolved in methanol to prepare 50 ml of stock solution in concentration of 500 ppm that served as standard antioxidant. In the same way, 50 ml of separate stock solutions were prepared for every test sample of Ru.Cr and Ru.F. Final concentrations of 40, 60, 80, 100 and 200 ppm (5 ml each test samples) were prepared in test tubes through serial dilutions. To each test tube, 1ml of stable DPPH was added. The test tubes were incubated in dark for 30 min at room temperature. Absorbances of test solutions were measured at 517 nm using UV spectrophotometer (Shimadzu UV-1700). Methanol was used as blank. Percent inhibition or free radical scavenging of DPPH was determined using following formula:

$$ \%\ \mathrm{inhibition}\ \mathrm{of}\ \mathrm{DPPH}=\frac{\mathrm{Absorbance}\ \mathrm{of}\ \mathrm{control}\hbox{-} \mathrm{Absorbance}\ \mathrm{of}\ \mathrm{sample}}{\mathrm{Absorbance}\ \mathrm{of}\ \mathrm{control}}\times 100 $$

Antipyretic activity

Pyrexia was induced in rats by injecting 20% (20 g of dried Brewer’s yeast in 100 ml of 2% gum acacia and 0.9% saline aqueous suspension) of Brewer’s yeast in a dose of 1 ml/100 g body weight (s.c) [45]. The animals were fasted for 18 h. They had free access to water. Digital thermometer was used to record rectal temperature of test animals. Rats with a temperature rise of at least 0.5 °C to 1 °C were included in the study. Animals were divided into six groups (GI - GVI) having 6 rats in each group. The GI served as negative control group, which received 2% gum acacia (p.o). GII represented the standard group and received paracetamol (33 mg/kg of body weight in 2% gum acacia) (p.o). GIII and GIV received 150 mg/kg and 300 mg/kg body weight of Ru.Cr (p.o). While GV and GVI received 150 mg/kg and 300 mg/kg body weight of Ru.F (p.o), respectively. Rectal temperature of the animals was noted on an hour interval for 4 h following the administration of paracetamol and the test samples. Decrease in rectal body temperature of test animals was calculated by the following formula [46]:

$$ \mathrm{Percentage}\ \mathrm{reduction}\ \mathrm{in}\ \mathrm{pyrexia}=\frac{\mathrm{B}\hbox{-} \mathrm{C}n}{\mathrm{B}\hbox{-} \mathrm{A}}\times 100 $$

= Normal body temperature.


= Rectal temperature at 18th h after yeast administration.

Cn :

= Rectal temperature after administration of drug.

Results and discussion

Preliminary phytochemical screening

Results for the presence of different phytochemicals are mentioned in Table 1. It tested positive for the presence of flavonoids, alkaloids, tannins, saponins, glycosides, phenolic compounds, sterols, carbohydrates, terpenoids and proteins.

Table 1 Results of different phytochemicals present in Rubus ulmifolius

Acute toxicity studies

The purpose of acute toxicity studies is to determine a safe dose range which can be extrapolated to human model [47]. No death toll was observed till the maximum oral dose (6000 mg/kg) of Ru.Cr and Ru.F in all test groups. These results suggest that Ru.Cr and Ru.F are safe up to 6000 mg/kg body weight per oral route. Because of high margin of safety in acute toxicity study, we selected 150 mg/kg and 300 mg/kg oral doses for possible in vivo antipyretic activity.

Brine shrimp cytotoxicity study

The effects of Ru.Cr and Ru.F on brine shrimps for possible lethality are shown in Table 2.

Table 2 Brine shrimp cytotoxicity assay of test samples of Rubus ulmifolius

The magnitude of lethality was directly proportional to the concentration of test samples. Ru.Cr has an LC50 of 16.7 ± 1.4 μg/ml. LCmax∞100% was in concentration of 100 μg/ml. Similarly, Ru.F has an LC50 of 10.6 ± 1.8 μg/ml with maximum mortality in concentration of 75 μg/ml i.e. LCmax∞100%. This explains that the flavonoids rich extract may have cytotoxic constituents as well. This study justifies that the plant may be a potential source for isolation of anticancer bioactive molecules as there are reports that correlate simple bench‐top bioassays and human tumour cell cytotoxicities as potential antitumor agents [48, 49]. This needs for isolation of bioactive molecules as plants are good sources of anticancer drugs.

Antioxidant activity

Results of antioxidant activity of Ru.Cr and Ru.F are shown in Table 3.

Table 3 Antioxidant activity of Rubus ulmifolius

Ru.Cr showed 59.4 ± 0.073% inhibition in concentration of 40 ppm and 74.2 ± 0.324% inhibition in concentration of 200 ppm. Percent scavenging activity of ascorbic acid was 87.26 ± 0.128%. Free radical scavenging with Ru.F is 61.43 ± 0.256% in concentration of 40 ppm. Ascorbic acid inhibited the free radical scavenging activity up to 62.7 ± 0.291%. More, in highest concentration (200 ppm), Ru.F showed 83.16 ± 0.091% free radical scavenging activity. While ascorbic acid demonstrated 87.26 ± 0.128% free radical scavenging activity. This shows that the free radical scavenging activity of Ru.F is comparable with ascorbic acid, a standard antioxidant.

In basic process of oxidation, the most important step is the formation of free radicals (having unpaired electrons) in living systems, drugs and even food [50]. Free radicals, which are extremely unstable, causes many diseases of kidney and liver, Alzheimer’s disease, fibrosis, cardiac reperfusion abnormalities, atherosclerosis, cancer, arthritis, neurodegenerative disorders and aging [51].

It is reported that antioxidants prevent the oxidative damage which is caused by reactive oxygen species (ROS) and free radicals [52]. Antioxidants act by interfering in the oxidation process reaction with the chelating, catalytic metals and free radicals as oxygen scavengers [53, 54]. Ru.Cr and Ru.F produced comparable free radical scavenging activities both in low and high concentrations versus standard ascorbic acid. The order of free radical scavenging of test samples (40 and 200ppm) and standard is: ascorbic acid > Ru. F > Ru.Cr. Our findings confirm reports that Rubus species are extremely abundant with phenolic contents like flavonoids, phenolic acids and anthocyanins. These phenolic compounds have been reported to possess antioxidant, anti-inflammatory and anticancer properties [55]. Amongst different species of genus Rubus, Rubus ellipticus has exhibited antioxidant activities [56]. Rubus coreanus is reported to have antioxidant properties [57]. It has been reported that the phenolic compounds like flavonoids exhibited prominent antioxidant activities [58]. Based on reports for importance of phenolic components as antioxidants, the observed antioxidant activity may be attributed to the occurrence of flavonoids like active constituents which require further research for isolation of antioxidant components from the Rubus ulmifolius.

Antipyretic studies

Both Ru.Cr and Ru.F demonstrated significant antipyretic as mentioned in Table 4. Antipyretic effects are comparable with antipyretic effects of paracetamol, a standard antipyretic agent.

Table 4 Effect of crude and flavonoids extracts of Rubus ulmifolius on yeast-induced pyrexia in Rats

Ru.Cr in test dose of 300 mg reduced rectal temperature up to 74% on 4th hour of the administration. On 3rd hour, Ru.Cr decreased body temperature by 58%. Similarly, Ru.F produced 72% reduction in body temperature on 4th hour of the administration. Following 2nd hour of administration of Ru.F, decrease in body temperature was 59%, which is 52% of paracetamol, a standard antipyretic. This suggests that Ru.Cr and Ru.F have antipyretic activity. Antipyretic drugs are used to reduce the elevated body temperature both by depression of inflammatory mediators at sites of peripheral tissues, and at thermoregulatory sites within the Central Nervous System (CNS) [59]. NSAIDs are having antipyretic action due to their inhibitory effects on cyclooxygenase (COX), an enzyme responsible for production of prostaglandins, which are important mediators for producing pain and pyrexia. NSAIDs are said to possess antipyretic properties which resets the hypothalamic thermostat and thus reduces elevated body temperature during fever. Heat loss is promoted by cutaneous vasodilatation and sweating. Paracetamol produces anti-pyretic action while acting on CNS [60].

Yeast administration induces the release of phagocytes and interleukins (endogenous Pyrogens). Interleukins helps the production of T-lymphocytes, which in turn induce - hypothalamus to produce prostaglandins that finally elevate the body temperature [61].

In a test dose of 300 mg, it is evident that Ru.F produced 85.83% fall in body temperature. These results confirm that the crude methanolic extract and crude flavonoids rich extract of Rubus ulmifolius have significant antipyretic effect. This significant antipyretic effect of Rubus ulmifolius is attributed to the presence of phytochemicals like flavonoids, which are reported for inhibitory effects on cyclooxygenases [62, 63]. The flavonoids are known to act by blocking the synthesis of prostaglandins E2 predominantly through inhibition of prostaglandins synthetase enzyme [64]. Hence, it is deduced that the antipyretic effects may be attributed to flavonoids present in the species.

Thus, the present study confirms the folkloric use of Rubus ulmifolius as antipyretic remedy. However, further studies are suggested to isolate the pharmacologically active antipyretic constituents from the species.


The current work confirms that aerial parts of Rubus ulmifolius contain flavonoids that are safe up to 6g/kg (p.o). Crude methanolic extract and flavonoids rich fraction of Rubus ulmifolius have significant antioxidant and antipyretic activity. Further work is required to isolate the pharmacologically active substances for relatively safe and effective antipyretics and antioxidants.


LC50 :

Least concentration of a test sample that kills 50% of the nauplii

LCmax∞100% :

Least concentration of test sample that kills 100% of the nauplii. P.O per oral


Crude methanolic extract of Rubus ulmifolius


Extract of crude flavonoids rich fraction of Rubus ulmifolius


Sub cutaneous


  1. Ballabh B, Chaurasia O, Ahmed Z, Singh SB. Traditional medicinal plants of cold desert Ladakh—used against kidney and urinary disorders. J Ethnopharmacol. 2008;118(2):331–9.

    Article  PubMed  Google Scholar 

  2. Finn RD, Tate J, Mistry J, Coggill PC, Sammut SJ, Hotz H-R, Ceric G, Forslund K, Eddy SR, Sonnhammer EL. The Pfam protein families database. Nucleic Acids Res. 2008;36 suppl 1:D281–8.

    CAS  PubMed  Google Scholar 

  3. Hummer G. Molecular binding: Under water’s influence. Nat Chem. 2010;2(11):906–7.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  4. Patel-Schneider PF, Hayes P, Horrocks I. OWL web ontology language semantics and abstract syntax. W3C Recomm. Available on:

  5. Moyer A, Finney JW, Swearingen CE, Vergun P. Brief interventions for alcohol problems: a meta‐analytic review of controlled investigations in treatment‐seeking and non‐treatment‐seeking populations. Addiction. 2002;97(3):279–92.

    Article  PubMed  Google Scholar 

  6. Anthony J-P, Fyfe L, Stewart D, McDougall G. Differential effectiveness of berry polyphenols as anti-giardial agents. Parasitology. 2011;138(09):1110–6.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  7. Pradhan BK, Badola HK. Journal of ethnobiology and ethnomedicine. J Ethnobiol Ethnomed. 2008;4:22.

    Article  PubMed  PubMed Central  Google Scholar 

  8. Zhang Y, Zhang Z, Yang Y, Zu X, Guan D, Guan Y. Diuretic activity of Rubus idaeus L (Rosaceae) in rats. Trop J Pharm Res. 2011;10(3):243-248.

  9. Park P, Ko JW, Jeong C. Reciprocally convex approach to stability of systems with time-varying delays. Automatica. 2011;47(1):235–8.

    Article  Google Scholar 

  10. Shin TY, Kim SH, Lee ES, Eom DO, Kim HM. Action of Rubus coreanus extract on systemic and local anaphylaxis. Phytother Res. 2002;16(6):508–13.

    Article  PubMed  Google Scholar 

  11. Ju HK, Cho EJ, Jang MH, Lee YY, Hong SS, Park JH, Kwon SW. Characterization of increased phenolic compounds from fermented Bokbunja (Rubus coreanus Miq.) and related antioxidant activity. J Pharm Biomed Anal. 2009;49(3):820–7.

    CAS  Article  PubMed  Google Scholar 

  12. Park Y, Chang H. Lactic acid fermentation and biological activities of Rubus coreanus. Applied Biological Chemistry. 2003;46(4):367-375.

  13. Kim EJ, Lee Y-J, Shin H-K, Park JHY. Induction of apoptosis by the aqueous extract of Rubus coreanum in HT-29 human colon cancer cells. Nutrition. 2005;21(11):1141–8.

    Article  PubMed  Google Scholar 

  14. Sher H. Ethnoecological evaluation of some medicinal and aromatic plants of Kot Malakand Agency. Pak Sci Res Essays. 2011;6(10):2164–73.

    Article  Google Scholar 

  15. Manganelli RU, Tomei P. Ethnopharmacobotanical studies of the Tuscan Archipelago. J Ethnopharmacol. 1999;65(3):181–202.

    Article  Google Scholar 

  16. Ahmad N, Anwar S, Fazal H, Abbasi BH. Medicinal plants used in indigenous herapy by people of Madyan Valley in district Swat, Pakistan. Int J Med Aromat Plants. 2013;3(1):47–54.

    Google Scholar 

  17. Lemus I, Garcia R, Delvillar E, Knop G. Hypoglycaemic activity of four plants used in Chilean popular medicine. Phytother Res. 1999;13(2):91–4.

    CAS  Article  PubMed  Google Scholar 

  18. Wada L, Ou B. Antioxidant activity and phenolic content of Oregon caneberries. J Agric Food Chem. 2002;50(12):3495–500.

    CAS  Article  PubMed  Google Scholar 

  19. Panizzi L, Caponi C, Catalano S, Cioni P, Morelli I. In vitro antimicrobial activity of extracts and isolated constituents of Rubus ulmifolius. J Ethnopharmacol. 2002;79(2):165–8.

    CAS  Article  PubMed  Google Scholar 

  20. Begum TN, Ilyas MHM, Anand AV. Antipyretic activity of azima tetracantha in experimental animals. Int J Cur Biomed Phar Res. 2011;1(2):41–4.

    Google Scholar 

  21. Cook N, Samman S. Flavonoids—chemistry, metabolism, cardioprotective effects, and dietary sources. J Nutr Biochem. 1996;7(2):66–76.

    CAS  Article  Google Scholar 

  22. Narayana KR, Reddy MS, Chaluvadi M, Krishna D. Bioflavonoids classification, pharmacological, biochemical effects and therapeutic potential. Indian J Pharmacol. 2001;33(1):2–16.

    CAS  Google Scholar 

  23. O’Prey J, Brown J, Fleming J, Harrison PR. Effects of dietary flavonoids on major signal transduction pathways in human epithelial cells. Biochem Pharmacol. 2003;66(11):2075–88.

    Article  PubMed  Google Scholar 

  24. Fernández SP, Wasowski C, Loscalzo LM, Granger RE, Johnston GA, Paladini AC, Marder M. Central nervous system depressant action of flavonoid glycosides. Eur J Pharmacol. 2006;539(3):168–76.

    Article  PubMed  Google Scholar 

  25. Heim KE, Tagliaferro AR, Bobilya DJ. Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. J Nutr Biochem. 2002;13(10):572–84.

    CAS  Article  PubMed  Google Scholar 

  26. Cazarolli LH, Zanatta L, Alberton EH, Figueiredo B, Reis MS, Folador P, Damazio RG, Pizzolatti MG, Silva B, Mena FR. Flavonoids: prospective drug candidates. Mini Rev Med Chem. 2008;8(13):1429–40.

    CAS  Article  PubMed  Google Scholar 

  27. Guardia T, Rotelli AE, Juarez AO, Pelzer LE. Anti-inflammatory properties of plant flavonoids. Effects of rutin, quercetin and hesperidin on adjuvant arthritis in rat. Il Farmaco. 2001;56(9):683–7.

    CAS  Article  PubMed  Google Scholar 

  28. Rotelli AE, Guardia T, Juárez AO, De la Rocha NE, Pelzer LE. Comparative study of flavonoids in experimental models of inflammation. Pharmacol Res. 2003;48(6):601–6.

    CAS  Article  PubMed  Google Scholar 

  29. Bıtıs L, Kultur S, Melıkoglu G, Ozsoy N, Can A. Flavonoids and antioxidant activity of Rosa agrestis leaves. Nat Prod Res. 2010;24(6):580–9.

    Article  PubMed  Google Scholar 

  30. Pal RS, Ariharasivakumar G, Girhepunjhe K, Upadhay A. In-vitro antioxidative activity of phenolic and flavonoids compounds extracted from seeds of Abrus precatorius. Int J Pharm Pharm Sci. 2009;1(2):136–40.

    CAS  Google Scholar 

  31. Havsteen BH. The biochemistry and medical significance of the flavonoids. Pharmacol Ther. 2002;96(2):67–202.

    CAS  Article  PubMed  Google Scholar 

  32. Sharififar F, Dehghn-Nudeh G, Mirtajaldini M. Major flavonoids with antioxidant activity from < i > Teucrium polium</i > L. Food Chem. 2009;112(4):885–8.

    CAS  Article  Google Scholar 

  33. Boots AW, Haenen GR, Bast A. Health effects of quercetin: from antioxidant to nutraceutical. Eur J Pharmacol. 2008;585(2):325–37.

    CAS  Article  PubMed  Google Scholar 

  34. Zayachkivska O, Konturek S, Drozdowicz D, Konturek P, Brzozowski T, Ghegotsky M. Gastroprotective effects of flavonoids in plant extracts. J Physiol Pharmacol Suppl. 2005;56(1):219–31.

    Google Scholar 

  35. Lee JH, Kim GH. Evaluation of antioxidant and inhibitory activities for different subclasses flavonoids on enzymes for rheumatoid arthritis. J Food Sci. 2010;75(7):H212–7.

    CAS  Article  PubMed  Google Scholar 

  36. Tapas A, Sakarkar D, Kakde R. Flavonoids as nutraceuticals: a review. Trop J Pharm Res. 2008;7(3):1089–99.

    Article  Google Scholar 

  37. Lin J, Weng M-S. Flavonoids as nutraceuticals. In: The science of flavonoids. Springer New York; 2006. 213–238.

  38. Halliwell B, Rafter J, Jenner A. Health promotion by flavonoids, tocopherols, tocotrienols, and other phenols: direct or indirect effects? Antioxidant or not? Am J Clin Nutr. 2005;81(1):268S–76S.

    CAS  PubMed  Google Scholar 

  39. Harborne JB, Grayer RJ. The anthocyanins. In: The flavonoids. Springer US; 1988. 1–20.

  40. Evans WC. Trease and Evans’ pharmacognosy. Elsevier Health Sciences UK; 2009.

  41. Sofowora A. Recent trends in research into African medicinal plants. J Ethnopharmacol. 1993;38(2):197–208.

    Article  Google Scholar 

  42. Lorke D. A new approach to practical acute toxicity testing. Arch Toxicol. 1983;54(4):275–87.

    CAS  Article  PubMed  Google Scholar 

  43. Ali N, Shah SW, Shah I, Ahmed G, Ghias M, Khan I. Cytotoxic and anthelmintic potential of crude saponins isolated from Achillea Wilhelmsii C. Koch and Teucrium Stocksianum boiss. BMC Complement Altern Med. 2011;11(1):106.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  44. Ilahi I, Samar S, Khan I, Ahmad I. Search types. Pak J Pharm Sci. 2013;26:949.

    PubMed  Google Scholar 

  45. Mandal S, Upadhyay N, Sharma I. A comparative antipyretic activity of the crude extracts of the ariel parts of Glycosmis pentaphylla and Bauhinia variegate. Recent Res Sci Technol. 2011;3(7):16–8.

    Google Scholar 

  46. Lekshmi UD, Reddy PN. Preliminary studies on antiinflammatory, antipyretic, and antidiarrhoeal properties of Evolvulus alsinoides. Turk J Biol. 2011;35(5):611–8.

    Google Scholar 

  47. Zahra A, Kadir F, Mahmood A, Suzy S, Sabri S, Latif I, Ketuly K. Acute toxicity study and wound healing potential of Gynura procumbens leaf extract in rats. J Med Plants Res. 2011;5(12):2551–8.

    Google Scholar 

  48. Sökmen A. Antiviral and cytotoxic activities of extracts from the cell cultures and respective parts of some Turkish medicinal plants. Turk J Biol. 2001;25:343–50.

    Google Scholar 

  49. Anderson J, Goetz C, McLaughlin J, Suffness M. A blind comparison of simple bench‐top bioassays and human tumour cell cytotoxicities as antitumor prescreens. Phytochem Anal. 1991;2(3):107–11.

    CAS  Article  Google Scholar 

  50. Pourmorad F, Hosseinimehr S, Shahabimajd N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr J Biotechnol. 2006;5(11):1142–45.

  51. Kang M-C, Kim SY, Kim YT, Kim E-A, Lee S-H, Ko S-C, Wijesinghe W, Samarakoon KW, Kim Y-S, Cho JH. In vitro and in vivo antioxidant activities of polysaccharide purified from aloe vera (Aloe barbadensis) gel. Carbohydr Polym. 2014;99:365–71.

    CAS  Article  PubMed  Google Scholar 

  52. Leong CNA, Tako M, Hanashiro I, Tamaki H. Antioxidant flavonoid glycosides from the leaves of Ficus pumila L. Food Chem. 2008;109(2):415–20.

    CAS  Article  PubMed  Google Scholar 

  53. Shahidi F, Janitha P, Wanasundara P. Phenolic antioxidants. Crit Rev Food Sci Nutr. 1992;32(1):67–103.

    CAS  Article  PubMed  Google Scholar 

  54. Güder A, Korkmaz H. Evaluation of in-vitro antioxidant properties of hydroalcoholic solution extracts Urtica dioica L., Malva neglecta Wallr. and their mixture. Iran J Pharm Res. 2012;11(3):913.

    PubMed  PubMed Central  Google Scholar 

  55. Sariburun E, Şahin S, Demir C, Türkben C, Uylaşer V. Phenolic content and antioxidant activity of raspberry and blackberry cultivars. J Food Sci. 2010;75(4):C328–35.

    CAS  Article  PubMed  Google Scholar 

  56. Vadivelan R, Bhadra S, Ravi A, Shanish A, Elango K, Suresh B. Evaluation of anti-inflammatory and membrane stabilizing property of ethanol root extract of Rubus ellipticus Smith in Albino rats. J Nat Rem. 2009;9(1):74–8.

    Google Scholar 

  57. Choi S-J, Lee Y-S, Kim J-K, Chung C-K, Kang I-J, Lim S-S. Antioxidant activity of ripe fruit components of Rubus coreanus: extraction using porous polymer resins. Korean J Food Sci Technol. 2011;43(2):149–55.

    Article  Google Scholar 

  58. Amjad L, Shafighi M. Evaluation of antioxidant activity, phenolic and flavonoid content in Punica granatum var. Isfahan malas flowers. Int J Agric Crop Sci. 2013;5(10):1133.

    Google Scholar 

  59. Aronoff DM, Neilson EG. Antipyretics: mechanisms of action and clinical use in fever suppression. Am J Med. 2001;111(4):304–15.

    CAS  Article  PubMed  Google Scholar 

  60. Aronoff DM, Oates JA, Boutaud O. New insights into the mechanism of action of acetaminophen: Its clinical pharmacologic characteristics reflect its inhibition of the two prostaglandin H2 synthases. Clinical Pharmacol Ther. 2006;79(1):9–19.

    CAS  Article  Google Scholar 

  61. Earle RR, Subramanyam PV. A review on microneedles: an emerging transdermal drug delivery system. Indo AM J Pharm Res. 2014;4:1917–22.

    Google Scholar 

  62. Mutalik S, Paridhavi K, Rao CM, Udupa N. Antipyretic and analgesic effect of leaves of Solanum melongena Linn. In rodents. Indian J Pharmacol. 2003;35(5):312–5.

    Google Scholar 

  63. Rust G, Ye J, Baltrus P, Daniels E, Adesunloye B, Fryer GE. Practical barriers to timely primary care access: impact on adult use of emergency department services. Arch Intern Med. 2008;168(15):1705–10.

    Article  PubMed  Google Scholar 

  64. Levine D, Lubensky T, Ostlund S, Ramaswamy S, Joseph Steinhardt P, Toner J. Elasticity and dislocations in pentagonal and icosahedral quasicrystals. Phys Rev Lett. 1985;54(14):1520–3.

    CAS  Article  PubMed  Google Scholar 

Download references


The authors are thankful to Dr. Nasrullah, University of Malakand for identification of the plant species.


No fund was available this project as it was a self-funded project.

Availability of data and materials

All data generated or analyzed during this study are included in this published article.

Authors’ contributions

NA: Supervision of the pharmacological tests and critical revision of the manuscript. MS: Supervision of the research work. SWAS: interpretation of some laboratory data and wrote the introduction section. IS: manuscript preparation. MS: Performed the pharmacological tests (M.Phil Scholar). All authors read and approved the final manuscript.

Authors’ information

As provided in the abstract section.

Competing interests

The authors declare that they have no competing interests.

Consent for publication

Not applicable.

Ethics approval and consent to participate

Approval was obtained from the Ethics board of the Khyber Medical University, Peshawar, Pakistan. Consent is not applicable.

Author information

Authors and Affiliations


Corresponding author

Correspondence to Niaz Ali.

Rights and permissions

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Ali, N., Shaoib, M., Shah, S.W.A. et al. Pharmacological profile of the aerial parts of Rubus ulmifolius Schott. BMC Complement Altern Med 17, 59 (2017).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI:


  • Rubus ulmifolius
  • Antipyretic
  • Paracetamol
  • Flavonoids
  • Brewer’s yeast
  • Pyrexia
  • Antioxidant