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Penthorum chinense Pursh improves type 2 diabetes mellitus via modulating gut microbiota in db/db mice

BMC Complementary Medicine and Therapies volume 23, Article number: 314 (2023) Cite this article

Abstract

Penthorum chinense Pursh (P. chinense) has been traditionally used as hepatoprotective food and medicine for hundreds of years due to its significant antioxidant and anti-inflammatory activities. However, the efficacy and mechanisms of action of P. chinense in type 2 diabetes mellitus were not fully understood. In this study, we found that P. chinense extract (PCP) supplementation resulted in reduced body weight and hyperglycemia, improved pancreatic tissue injury and insulin sensitivity, and decreased inflammatory cytokines expression in spontaneously diabetic db/db mice. 16S rRNA gene sequencing of fecal samples showed that PCP administration decreased the abundance of Firmicutes and increased the proportion of Bacteroidetes at the phylum level. Moreover, Muribaculum, Barnesiella, Prevotella, and Mucinivorans were enriched, with Desulfovibrio and Lactobacillus lowered at the genus level in db/db mice with PCP supplementation. These results suggested that PCP may ameliorate hyperglycemia, insulin resistance, and inflammation by remodeling the gut microbiota in db/db mice.

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Introduction

The latest data published by the International Diabetes Federation in 2023 shows that there are approximately 537 million people worldwide suffering from diabetes, with type 2 diabetes (T2DM) accounting for over 90% of these cases. T2DM is a chronic metabolic disease manifested as hyperglycemia with insulin resistance (IR) or relatively inadequate insulin secretion [1]. However, the underlying mechanism and effective prevention/treatment for T2DM are still not fully discovered. As a progressive disease, systemic inflammation and metabolic disruption contribute to various complications caused by abnormal blood glucose. Hence, it is critical to maintain glucose homeostasis in individuals with T2DM.

The gut has been recognized as the biggest “immune organ” for years, and the intestine microbiome living inside was altered by disease, drugs, diet, and environment [2]. The potential role of gut microbiota exhibited in the pathogenesis and progression of T2DM has been reported in both animal and human studies [3, 4]. Significant changes in the structure and composition of the intestinal flora of patients with T2DM, characterized by a decrease in the abundance of probiotic bacteria, such as Bifidobacterium and Roseburia, and an increase in the abundance of opportunistic pathogens, including Escherichia coli and Desulfovibrio [5]. The gut microbiota can produce short-chain fatty acids (SCFA), lipopolysaccharides (LPS), secondary bile acids (BA), peptidoglycan, and a variety of other beneficial or harmful metabolites, which in turn act on the host. SCFA can regulate the gut-liver axis to maintain glucose homeostasis and reduce IR, inhibit inflammation, and repair the intestinal barrier [6, 7]. LPS promotes metabolic endotoxemia and low-grade inflammation, which exacerbate IR and T2DM [8]. BA activates receptors including farnesoid X receptor (FXR) and G protein-coupled bile acid receptor 5 (TGR5) to trigger downstream cascades to regulate glucose metabolism [9,10,11]. Modulation of gut microbiota is increasingly believed as a promising approach for the treatment of T2DM.

Penthorum chinense Pursh (P. chinense) has been used as a medicinal and dietary herb in China for hundreds of years. It was demonstrated by infrared spectroscopy that polyphenols and flavonoids (kaempferol, quercetin, and pinocembrin-7-O-beta-D-glucoside) were the most abundant biomolecules in P. chinense extract (PCP) [12,13,14]. Notably, polyphenol-rich herbal medicine and functional food like green tea, Pueraria lobata and Moringa oleifera have long been known to offer diabetes-protective properties [15,16,17]. Previous study had reported that PCP activated the Nrf2 antioxidant pathway by binding to Keap1 protein, and significantly reduced the vascular inflammation induced by high glucose levels [18]. Huang et al. [19] found that polyphenols of PCP possessed a modest hypoglycemic effect by increasing insulin secretion on diabetic rats induced by high-fat diet (HFD) combine with streptozotocin (STZ). Meanwhile, PCP had been reported to inhibit intestinal inflammation in animals, enhance the α diversity of the gut microbiota, and enrich SCFA-producing bacteria [20,21,22]. However, there is no report about the hypoglycemic effects of PCP in db/db mice, and the underlying hypoglycemic mechanism of PCP is still unclear. In addition, the role of gut microbiota in the therapeutic efficacy of PCP in T2DM remains to be further investigated.

The present study attempted to investigate the role of gut microbiota in PCP protecting db/db mice from T2DM. We have examined glucose- and lipid-related parameters, inflammatory cytokines, and pathological changes in the pancreas to determine the hypoglycemic effect of PCP. Changes in gut microbiota were analyzed by 16S rRNA sequencing to further identify the relationship among PCP, gut microbiota, and T2DM. The current study may provide a new perspective on the mechanism of PCP in the treatment of T2DM.

Materials and methods

Herbal materials and Preparation

The dried P. chinense was obtained from Gulin Hongan Pharmaceutical Co., Ltd. (Luzhou, China). Samples were ground into powder and adequately immersed in ultrapure water. Then, the mixture was exhaustively extracted with boiling water three times for 1 h each. Finally, the resulting decoction was concentrated to 1 g/mL.

Animals experiment

All animal experiments were approved by the Ethics Committee of Southwest Medical University (No.201910-236). The male db/db and db/m mice (8 weeks) were provided by Changzhou Cavens Co., Ltd. (Changzhou, China). All animals were fed in the SPF Animal Laboratory Center of Southwest Medical University at 12 h light/dark cycle, (23 ± 2) ℃, and (60 ± 5) % relative humidity. All mice accessed food and water freely. After seven days of acclimatization, the fasting blood glucose (FBG) was monitored by a glucometer and glucose test strips (Roche Co., Germany). The db/db mice detected with FBG over 11.1 mmol/L were defined as diabetic mice. Eight db/m mice were involved in the normal control group (NC) and twenty-four db/db mice were randomly divided into three groups: diabetic model control group (DC), metformin group (Met), and PCP treatment group (PCP). The Met group received metformin 150 mg/kg/d (Glucophage, manufactured by Sino-American Shanghai Squibb Pharmaceuticals Ltd.). The PCP group was administered a dose of 1200 mg/kg/d, which was determined according to our earlier pre-experimental results. Allometric scaling to a person with a body weight of 60 kg, the dose corresponds to 8-gram PCP daily, at the tolerable upper intake levels of humans. Only ultrapure water was given to the animals in the NC and DC. All treatments were administered by gavage for four weeks.

Oral glucose tolerance test

All animals were given 50% glucose solution at a dose of 2 g/kg when OGTT was conducted, and tail venous blood was collected at 0, 30th, 60th, and 120th minutes after oral gavage to measure blood glucose concentration.

Biochemical analyses

Mice were terminally anesthetized with 1% sodium pentobarbital solution (1 mL/100 g) and collected blood samples and viscera. 1 mL of blood samples was centrifuged at 3500 rpm for 10 min to obtain serum samples, and 0.5 mL of whole blood was centrifuged at 10,000 rpm for 10 min to collect hemocytes for the measurement of glycated hemoglobin A1c (HbA1c). Serum triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterin (LDL-C), and high-density lipoprotein cholesterin (HDL-C) were determined by commercial kits (Jiancheng Co., Ltd., Nanjing, China). Fasting serum insulin (FINS), HbA1c, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were detected by ELISA kits (Meilian Co., Ltd., Shanghai, China). IR is indicated by homeostasis model assessment of insulin resistance (HOMA-IR) with a calculation formula of FBG (mmol/L) × FINS (mU/L)/22.5.

Pancreas tissue histopathological analysis

The pancreas tissue was prefixed with 2.5% glutaraldehyde solution and fixed with 1% osmium tetroxide solution. The tissue was dehydrated with acetones by gradient, permeated, embedded, cut into approximately 50 nm-thick lice, and stained with uranyl acetate and lead citrate solution. The pancreas slices were observed under transmission electron microscopy (TEM, JEM-1400PLUS, JEOL, Japan).

Gut microbiota analysis

Randomly selected five mice from each group to collect fecal samples at the end of the intervention. Straightway froze the samples in liquid nitrogen before being preserved at -80 ℃. Fecal bacteria DNA was extracted by the DP4015 fecal genomic DNA extraction kits (Pacific Biosciences Co., California, USA). The purity and concentration of the extracted fecal were measured, and the Illumina MiSeq third-generation sequencing was provided by Wuhan Hope Group Biotechnology Co., Ltd. The raw data was mass filtered and connected with double-ended sequences, and the high-quality sequence was screened. The clustering and annotation of OTU were performed to analyze the diversity index, the community structure, and species abundance at each taxonomic level.

Statistical analysis

All data were analyzed by SPSS 22.0 statistical software and the results are presented as the mean ± standard deviation (SD). One-way ANOVA analysis was performed to compare the mean differences among multiple groups. In pairwise comparison, the LSD test was used for those with homogeneous variances, and the Dunnett T3 test was used for those with uneven variances. The correlation between the gut microbiota and physicochemical indicators was calculated by Spearman correlation analysis. A P-value of less than 0.05 was deemed statistically significant.

Results

PCP inhibited the increase in body and organ weights in db/db mice

As shown in Fig. 1, the body, pancreas and liver weights as well as liver ratio of db/db mice were significantly higher than those of db/m mice (P < 0.05, P < 0.001). After 4 weeks of Met treatment, a significant decrease of the liver and liver ratio, was observed in Met group (P < 0.05, P < 0.01). After 4 weeks of PCP administration, the body weight, liver, and pancreas weights of PCP group were significantly reduced (P < 0.01, P < 0.001).

The ultrastructure of the pancreatic tissue was observed by TEM (Fig. 1E), which revealed intact nuclear membranes, a large number of β granules, and well-defined organelles including mitochondria, rough endoplasmic reticulum and ribosomes in islet β cells of db/m mice. There was a decrease in β granules among islet β cells of the DC group when compared to the NC group, while the cytoplasm contained large amounts of α particles with some mitochondria slightly swollen, and most of the rough endoplasmic reticulum dilated in a cystic shape. The β granules in islet β cells were augmented in the Met and PCP groups in comparison to that of the DC group, with comparable improvements in both groups. After treatment with Met and PCP, rough endoplasmic reticulum expansion in islet β cells of db/db mice was significantly improved and mitochondrial swelling was amended to varying degrees.

Fig. 1
figure 1

Effect of PCP on body and organ weights in db/db mice. (A) Body weight, (B) Pancreas weight, (C) Liver weight, (D) Liver ratio and (E) representative photomicrographs of TEM observation of pancreatic ultrastructure (scale bar, 500 nm). The purple arrows (↑) pointed to pancreatic β cells with abundant β granules, the green arrows (↑) pointed to pancreatic α cells with abundant α granules, the red arrows (↑) displayed the location of mitochondrial swelling, and the blue arrows (↑) displayed the location of endoplasmic reticulum expansion. All data are presented as the mean ± SD (n = 8). #, ##, and ### indicate P < 0.05, P < 0.01, and P < 0.001, respectively, in the comparison between the NC and DC groups. *, **, and ***indicate P < 0.05, P < 0.01, and P < 0.001, respectively, in comparisons between the DC group and the treatment groups

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PCP improved FBG, FINS and glucose tolerance in db/db mice

As described in Fig. 2A-D, db/db mice exhibited significant hyperglycemia and hyperinsulinemia compared to db/m mice (P < 0.001). FBG in db/db mice was reduced to varying degrees after treatment with metformin and PCP (P < 0.001). The levels of HbA1c in db/db mice were lowered by 37.41% and 35.69% respectively, but without significant differences compared to the DC group (P > 0.05). FINS and HOMA-IR were significantly lower in db/db mice after administration of metformin and PCP (P < 0.001, P < 0.05).

The results of OGTT were shown in Fig. 2E-F. The blood glucose values and AUC of the db/db mice were significantly higher than those of the db/m mice at all time points (P < 0.001). After four weeks of treatment with metformin, the blood glucose values and AUC of the Met group were significantly lower than those of the DC group at all time points (P < 0.05, P < 0.01, P < 0.001). Blood glucose values at 60th and 120th minutes and AUC of the PCP group were significantly lower than those of the DC group (P < 0.001).

Fig. 2
figure 2

Effect of PCP on blood glucose, insulin, and glucose tolerance in db/db mice. (A) FBG, (B) HbA1c, (C) FINS, (D) HOMA-IR, (E) The curve of OGTT and (F) AUC of OGTT. All data are presented as the mean ± SD (n = 8). #, ##, and ### indicate P < 0.05, P < 0.01, and P < 0.001, respectively, in the comparison between the NC and DC groups. *, **, and ***indicate P < 0.05, P < 0.01, and P < 0.001, respectively, in comparisons between the DC group and the treatment groups

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PCP decreased blood lipid levels in db/db mice

Serum TC, TG, and LDL-C levels were significantly elevated and HDL-C levels were significantly depressed in db/db mice when compared to db/m mice (Fig. 3, P < 0.001). After treatment with Met and PCP, the levels of TC, TG, and LDL-C in db/db mice were significantly decreased (P < 0.05, P < 0.01, P < 0.001). The levels of HDL-C in the Met group were effectively increased (P < 0.01), and those in the PCP group were raised to some extent but the difference was not statistically significant (P > 0.05).

Fig. 3
figure 3

Effect of PCP on blood lipid in db/db mice. (A) TG, (B) TC, (C) LDL-C, and (D) HDL-C. All data are presented as the mean ± SD (n = 8). #, ##, and ### indicate P < 0.05, P < 0.01, and P < 0.001, respectively, in the comparison between the NC and DC groups. *, **, and ***indicate P < 0.05, P < 0.01, and P < 0.001, respectively, in comparisons between the DC group and the treatment groups

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PCP attenuated inflammatory cytokine expression in db/db mice

As shown in Fig. 4, the contents of TNF-α and IL-6 were significantly higher in the DC group than in the NC group (P < 0.001). After treatment with Met and PCP, the expression of TNF-α and IL-6 was significantly decreased in the Met and PCP groups as compared to the DC group (P < 0.01, P < 0.001).

Fig. 4
figure 4

Effects of PCP on inflammatory cytokine in db/db mice. (A) TNF-α and (B) IL-6. All data are presented as the mean ± SD (n = 8). #, ##, and ### indicate P < 0.05, P < 0.01, and P < 0.001, respectively, in the comparison between the NC and DC groups. *, **, and ***indicate P < 0.05, P < 0.01, and P < 0.001, respectively, in comparisons between the DC group and the treatment groups

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PCP modified the diversity and richness of the gut microbiota in db/db mice

As shown in Fig. 5A-D, PD and Chao1 index were higher in db/db mice than those in db/m mice. In particular, PD was significantly raised in the Met and PCP groups than that in the NC group after metformin and PCP intervention (P < 0.05). A significantly higher Shannon index was observed in db/db mice as compared to db/m mice (P < 0.05). In parallel, the Simpson index of the Met group was significantly elevated in comparison to the NC group after treatment with metformin (P < 0.05).

Non-metric multidimensional scaling (NMDS) analysis showed a stress value of 0.12, where a stress value of less than 0.2 indicates reliable data results. NMDS analysis suggested noticeable differences in the composition of the microbial communities among the four groups. After treatment with PCP, the composition of the gut microbiota was closer to that of the NC group (Fig. 5E). The Venn diagrams showed that 7, 23, 13, and 31 unique OTUs were found in the NC, DC, Met, and PCP groups respectively, and 146 OTUs were identical across the four groups (Fig. 5F).

Fig. 5
figure 5

Effects of PCP on the diversity and richness of the gut microbiota. (A) Chao1 index, (B) PD index, (C) Shannon index, (D) Simpson, (E) NMDS analysis, and (F) Venn diagram based on comparisons of the overlapping and exclusive OTUs of the four groups. *, ** and *** indicate P < 0.05, P < 0.01 and P < 0.001, respectively

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PCP altered the composition of the intestinal flora in db/db mice

Firmicutes, Bacteroidetes, Proteobacteria, and Candidatus Saccharibacteria were the dominant microflora at the phylum level, especially Firmicutes and Bacteroidetes accounted for over 60% (Fig. 6A). Metastats analysis revealed that the relative abundance of Bacteroidetes was significantly decreased while the abundance of Proteobacteria was increased in the DC group when compared to the NC group (Fig. 6B, P < 0.05). These trends were effectively reversed by the administration of metformin and PCP. In the Met group, the relative abundance of Bacteroidetes and Verrucomicrobia increased while that of Proteobacteria decreased (P < 0.05, P < 0.01). Furthermore, the relative abundance of Bacteroidetes increased while that of Firmicutes decreased in the PCP group (P < 0.05).

As shown in Fig. 6C-D, the relative abundance of Alistipes, Muribaculum, Barnesiella, Mucinivorans, and Butyrivibrio markedly declined in the DC group compared to the NC group, while those of Desulfovibrio and Dialister significantly elevated at the genus level (P < 0.05, P < 0.01). In the Met group, the relative abundance of Muribaculum, Lachnoclostridium, Prevotella, Barnesiella, Flavonifractor, Ethanoligenens, and Butyrivibrio was significantly higher, while the relative abundance of Lactobacillus was significantly lower than those in the DC group (P < 0.05, P < 0.01). Compared with the DC group, six communities in the PCP group showed significant changes at the genus level, with significantly higher relative abundances of Muribaculum, Barnesiella, Prevotella, Mucinivorans, and lower relative abundances of Desulfovibrio and Lactobacillus (P < 0.05, P < 0.01). Notably, the relative abundance of Akkermansia, Alistipes, and Butyrivibrio was higher in the PCP group as compared to the DC group, but the difference was not statistically significant.

The dominant bacterial communities in each group were analyzed by linear discriminant analysis effect size (LEfSe), with a total of 45 biomarkers scoring more than 3 in linear discriminant analysis (LDA) (Fig. 6E-F). The superior genera of the NC group were Lactobacillus, Alistipes, Bifidobacterium, Turicibacter, and Odoribacter. The DC group was represented by Lacrimispora, Desulfovibrio, and Escherichia. The superior genera of the Met group were Lachnoclostridium, Muribaculum, Bacteroides, Prevotella, Akkermansia, Gordonibacter, Lachnospira, and Flavonifractor. The representative genera of the PCP group were Barnesiella and Mucinivorans.

Fig. 6
figure 6

Effects of PCP on the composition of the gut microbiota in db/db mice at phylum and genus levels. (A) Relative abundance of phylum level. (B) Metastats analysis at phylum level among NC, Met, PCP and DC groups. (C) Relative abundance of genus level. (D) Metastats analysis at genus level among NC, Met, PCP and DC groups. (E) Linear discriminant analysis. (F) LEfSe analysis. *, ** and *** indicate P < 0.05, P < 0.01 and P < 0.001, respectively

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Correlation between T2DM-related parameters and gut microbiota

As demonstrated in Fig. 7, the results of the correlation analysis between the top 20 high abundance genera and the diabetes-related indicators. Desulfovibrio and Escherichia showed positive correlations with FBG, HbA1C, body weight, TC, TG, LDL_C and IL-6 (P < 0.05, P < 0.01). Alistipes, Odoribacter, and Muribaculum showed strong positive correlations with the majority of glucose- and lipid-related indicators, inflammatory factors, and body weight were negatively correlated with them (P < 0.05, P < 0.01).

Fig. 7
figure 7

Heatmaps of the correlation between the T2DM-related parameters and the gut microbiota at the genus levels. *, ** and *** indicate P < 0.05, P < 0.01 and P < 0.001, respectively

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Discussion

T2DM is a metabolic disease characterized by chronic hyperglycemia with impaired insulin secretion and/or utilization. We evaluated the effects of PCP on blood glucose and lipids, IR, inflammatory cytokines, pancreatic tissue damage, and gut microbiota in db/db mice. It was found that PCP was effective in suppressing hyperglycemia, hyperinsulinemia, and the expression of inflammatory cytokines (IL-6 and TNF-α), and boosting glucose tolerance in db/db mice. These health-promoting effects of PCP may be related to the modulation of the structure and composition of the gut microbiota.

Growing evidence shows that dysbiosis of the gut microbiota is an underlying causative agent of T2DM and plays a potential regulatory role in its pathogenesis and progression [23]. The structure and composition of the intestinal flora play an essential role in the host’s health status. Previous studies have reported that PCP can significantly change the composition of the gut microbiota in healthy mice, common carp, and broilers by upregulating the relative abundance of Bacteroidetes and decreasing the amount of Firmicutes, contributing to a health-promoting intestinal environment [20, 21, 24]. Gut microbial changes characterized by a higher abundance of Firmicutes and a lower abundance of Bacteroidetes are frequently reported in patients with T2DM [25, 26]. Firmicutes are beneficial in stimulating host appetite and energy metabolism, and their abnormal increase may lead to obesity and IR in hosts. Bacteroidetes are considered to be the most common beneficial bacteria in the gut. The ratio of Firmicutes to Bacteroidetes is positively correlated with body weights and negatively associated with glucose tolerance [27]. In this study, PCP decreased the abundance of Firmicutes and increased the abundance of Bacteroidetes in db/db mice as expected, which was consistent with the trend in their body weight, lipids, blood glucose, and glucose tolerance. In addition, we found that PCP significantly raised the relative abundance of Mucinivorans at the genus level. It had been reported that Mucinivorans not only alleviated obesity and improved insulin sensitivity in hosts, but also regulated lipid metabolism and inhibited cholesterol synthesis [28].

Chronic inflammation induced by high glucose can lead to impaired islet β-cell function and worsen IR [29, 30]. As shown in the results, PCP treatment significantly decreased the expression of IL-6 and TNF-α as well as lowered the levels of FINS and HOMA-IR in db/db mice. It was further observed under TEM that PCP significantly improved pancreatic tissue edema and rough endoplasmic reticulum expansion and mitochondrial swelling of β cells, indicating that PCP could attenuate pancreatic injury and enhance insulin sensitivity in db/db mice by suppression of inflammation. Previous studies have shown that PCP can suppress inflammation in the body by activating the Nrf2/HO-1, AMPK/p62/Nrf2/mTOR, or SIRT1/AMPK signaling pathways [31,32,33]. Moreover, the gut microbiota can influence systemic metabolism and inflammatory states through the metabolism of nutrients and the release of anti- and pro-inflammatory factors. LPS is a major component of the cell wall of gram-negative bacteria and activates toll-like receptor 4 on the surface of macrophages to secrete pro-inflammatory cytokines upon entry into the bloodstream, causing endotoxemia [34]. In contrast, SCFA enhances barrier integrity by upregulating tight junction protein expression and increasing transmembrane resistance to reduce LPS entering the circulation and inhibit the expression of inflammatory factors [35]. Furthermore, as one of the most abundant microbial metabolites in the gut, SCFA has been shown to maintain glucose homeostasis and reduce host IR through the gut-pancreas-liver axis [36]. SCFA can increase insulin sensitivity and inhibit hepatic gluconeogenesis by binding to GPR41/43 on hepatic and pancreatic cell membranes to activate AMPK signal and cAMP-dependent protein kinase A leads to the phosphorylation of downstream target proteins [37].

A lack of SCFA-producing bacteria and an increase in LPS-producing bacteria are common manifestations of gut microbiota dysbiosis in patients with T2DM [38, 39]. In the present study, PCP treatment significantly decreased the relative abundance of Desulfovibrio and Lactobacillus, and increased the relative abundance of Muribaculum, Prevotella, and Barnesiella in db/db mice at the genus level. Desulfovibrio is the representative LPS-producing bacterium that converts sulfate into hydrogen sulfide, which disrupts the gut barrier and increases gut permeability [40]. Muribaculum and Prevotella are known to produce butyrate primarily, which is known to inhibit the expression of proinflammatory cytokine genes in adipocytes and contribute to preserving the integrity of the intestinal barrier [41, 42]. In addition, PCP was significantly enriched for the anti-inflammatory bacteria Barnesiella and Muribaculum. Barnesiella is associated with some immunomodulatory factors, such as IL-10, which can suppress inflammation in the gut [43, 44]. Ye’s study found that IL-10-/- mice with higher abundance of Barnesiella developed lower levels of colitis disease [45]. Moreover, Gao’ and Tao’ teams had proven that PCP treatment resulted in upregulating the expression of the anti-inflammatory factor IL-10 in the intestine of common carp and serum of dogs [20, 46], but further studies are needed to demonstrate whether it is associated with an increased abundance of Barnesiella in the gut. Muribaculum is an important mucin monosaccharide forager that inhibits Clostridium difficile colonization of the intestine, contributing to the reduction of intestinal inflammation and the maintenance of intestinal microecological stability [47]. In summary, we hypothesized that PCP suppressed intestinal inflammation and maintained intestinal homeostasis by increasing the abundance of SCFAs-producing and anti-inflammatory bacteria while decreasing the abundance of LPS-producing bacteria to ameliorated hyperglycemia and IR in db/db mice.

Notably, the relative abundance of Lactobacillus showed a significant decrease after PCP treatment, which may be contrary to some studies [48, 49]. Some ideas were that the increase of Lactobacillus, such as Lactobacillus johnsonii and Lactobacillus rhamnosus, can inhibit the growth of pathogens by secreting lactic acid to decrease the pH of the gut, maintain the intestinal barrier and repair the impaired glucose tolerance [50]. However, Karlsson et al. [51] had reported that Lactobacillus, classified to the phylum of Firmicutes, were notably enriched in T2DM individuals and positively correlated with FBG and HbA1c. Similarly, Li’s team proposed that PCP could alleviate dyslipidemia and IR via gut microbiota-bile acid-axis to reduce the abundance of bile salt hydrolase (BSH)-producing bacteria including Lactobacillus, Clostridium_IV and Clostridium_XIVb [22]. They also found that PCP treatment markedly decreased the protein levels of FXR in the ileum of HFD-fed mice and then downregulated the expression of fibroblast growth factor 15 (FGF15) due to the increased levels of taurine-conjugated BAs in feces. Taurine-bound muriatic acid present in mice is a natural antagonist of FXR [52]. FXR activated by taurine-conjugated BAs induces expression of FGF15, which promotes the uptake of chylomicrons by adipocytes through activation of the schizogenic-activated protein kinase pathway to promote adipocyte uptake of glucose to improve insulin sensitivity and glucose tolerance [53]. Moreover, He et al. [54] had revealed that Lactobacillus was mainly negatively associated with the expression levels of the liver PI3K/Akt/FoxO1 and GPR43/AMPK signaling pathways by correlation analysis. The PI3K/Akt/FoxO1 signaling cascade has been recognized as the principal mechanism of insulin signal transduction that plays a critical role in the modulation of hepatic IR [55]. Therefore, we conjectured whether the improvement of glycemia and IR in db/db mice by PCP was also associated with the gut-pancreas-liver axis. However, there was insufficient evidence for this assumption and further exploration will be needed.

The increased diversity of intestinal flora is beneficial for maintaining the stability of gut function [56]. Based on the results of α diversity analysis, we found that Chao1, PD, Simpson, and Shannon indexes were increased to some extent in db/db mice after PCP treatment. Interestingly, the PD and Shannon indexes were significantly lower in the NC group than those in the DC group. This was contrary to the results of previous studies, which overwhelmingly concluded that individuals with T2DM should have lower intestinal flora diversity than normal individuals [57]. Further analysis revealed that the results of Sun’s study were in agreement with ours, that db/db mice had higher α diversity of gut microbiota than db/m mice, and the experimental animals for both studies were from the same institution [58]. Therefore, we hypothesized that the results above may be related to the breeding environment and individual differences of animals.

To the best of our knowledge, this is the first time to investigate the effects of PCP on gut microbiota in db/db mice. Our findings provided a new perspective on the possible mechanism of PCP application in diabetes. However, there are some limitations to our study. Firstly, PCP, as a new novel food, was only approved to be consumed in the form of tea bags in China. Therefore, db/db mice were selected to be intervened with an aqueous extract of P. chinense to simulate the way of human consumption. Secondly, the biomarkers of PCP are not well-defined yet, and its active ingredients may act on different targets. Therefore, this study only describes the hypoglycemic effect of PCP at the level of the animal as a whole. In addition, whether the active ingredients of PCP exert hypoglycemic effects through the gut- pancreas-liver axis is a direction we would like to investigate deeply in the future.

Conclusion

In summary, our results demonstrated that PCP could ameliorate hyperglycemia, hyperlipidemia, IR, and expression of inflammatory cytokines in db/db mice by modulating the gut microbiota, which manifested by decreasing the abundance of LPS- and BSH-producing bacteria while enriching SCFA-producing and anti-inflammatory bacteria. Our findings provide a new basis for the application of PCP in diabetic treatment.

Availability of data and materials

All data generated or analyzed during this study are included in this published article. The raw data are available from the corresponding author on reasonable request.

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Funding

This research was funded by grants from the Key Research and Development of the Science and Technology Plan Project of Luzhou City (2018-NYF-17) and from the Joint Research Project of the General Hospital of Western Theater Command (KY2019006).

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Authors and Affiliations

  1. Clinical Nutrition, The General Hospital of Western Theater Command, Chengdu, 610083, P. R. China

    Jilei Hu & Ning Lin

  2. School of Public Health, Southwest Medical University, Luzhou, 646000, P. R. China

    Jilei Hu, Huibo Xie & Yan Yang

  3. Environmental health effects and risk assessment Key Laboratory of Luzhou, School of Public Health, Southwest Medical University, Luzhou, 646000, P. R. China

    Yan Yang

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Jilei Hu, Huibo Xie: Methodology, Investigation, Data curation, Writing Original draft preparation. Ning Lin *, Yan Yang *: Project administration, Supervision, Methodology, Financial Support, Writing-review & Editing.

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Hu, J., Xie, H., Lin, N. et al. Penthorum chinense Pursh improves type 2 diabetes mellitus via modulating gut microbiota in db/db mice. BMC Complement Med Ther 23, 314 (2023). https://doi.org/10.1186/s12906-023-04136-z

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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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