Fig. 2

Phosphorylation status of JAK2 (p-JAK2) and STAT3 (p-STAT3) together with their total protein level in tumor infiltrating immune cells, evaluated by Western blotting after administration of naringenin (50 mg/Kg/day) (Nar) and CPT (20 mg/kg/day) either individually or together (both at the concentration of 20 mg/kg/day). The blots were visualized on the X-ray film after processing of the blots with enhanced chemiluminescent (ECL) reagent. The original images are presented in supplementary file with the edges of the blot images visible (the blots were cut prior to hybridization). The results of the densitometry of the bands are presented in the graphs. Protein level of β-actin was used for normalizing the results. * P < 0.05, **P < 0.01, P < 0.001, compared to control. #P < 0.05 compared to CPT