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Fig. 5 | BMC Complementary Medicine and Therapies

Fig. 5

From: Chinese medicine Di-Huang-Yi-Zhi protects PC12 cells from H2O2-induced apoptosis by regulating ROS-ASK1-JNK/p38 MAPK signaling

Fig. 5

Effects of DHYZ on H2O2-induced ROS production. DHYZ and H2O2 treated PC12 cells were stained with DCFH-DA, observed under a fluorescence microscope (× 200) (A), detected by fluorescence microplate reader and expressed as fold of non-treated normal group (B). DHYZ treated PC12 cells were incubated with NAC for 2 h, followed by H2O2 treatment and subjected to ROS (C), Caspase-3 activity (D), apoptosis detection (E) and expressed as 100% of total (F). a, normal group; b, H2O2 group; c, control group; d, H2O2 + DHYZ (50 μg/ml) group; e, H2O2 + DHYZ (100 μg/ml) group; f, H2O2 + DHYZ (200 μg/ml) group. P < 0.01, versus normal group; *P < 0.05, **P < 0.01, ***P < 0.001, versus control group or versus NAC (−) group. The data are mean ± SD of three independent experiment each in triplicate

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