Fig. 5

Effects of DHYZ on H₂O₂-induced ROS production. DHYZ and H₂O₂ treated PC12 cells were stained with DCFH-DA, observed under a fluorescence microscope (× 200) (A), detected by fluorescence microplate reader and expressed as fold of non-treated normal group (B). DHYZ treated PC12 cells were incubated with NAC for 2 h, followed by H₂O₂ treatment and subjected to ROS (C), Caspase-3 activity (D), apoptosis detection (E) and expressed as 100% of total (F). a, normal group; b, H₂O₂ group; c, control group; d, H₂O₂ + DHYZ (50 μg/ml) group; e, H₂O₂ + DHYZ (100 μg/ml) group; f, H₂O₂ + DHYZ (200 μg/ml) group. ^△P < 0.01, versus normal group; ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, versus control group or versus NAC (−) group. The data are mean ± SD of three independent experiment each in triplicate