Fig. 3

Microarray analysis to identify the alteration in the gene expression and signal network. a Hierarchical gene clustering was performed with the TM4 Microarray Software Suite (MeV) for CME-treated SKOV-3 cells. Heat map revealed the genes that underwent more than twofold changes after apoptosis in response to CME treatment. The red and blue colors represent more than threefold change in the expression of upregulated and downregulated genes, respectively. The ratios of gene profiles are presented as heat map (left panel) and gene expression pattern (right panel). b Combined screenshots from the STRING website, showing the results obtained after entering a set of 12 proteins thought to be involved in the apoptotic signaling pathway. The insets are shown as follows (from top to bottom): the accessory information available for a single protein, reported enrichment of functional connections among the set of proteins, and statistical enrichments detected in functional subsystems. c One enriched function was selected, and the corresponding protein nodes in the network were automatically highlighted