Fig. 3

Effect of bloodletting puncture at HTWP on blood-brain barrier leakage at 5 h, 24 h, 72 h (n = 10 per group). a Representative images of brain slices stained by EB in sham-operated group, pMCAO group, bloodletting group. b Quantitative analysis of EB leakage. *P < 0.05, **P < 0.01 vs. sham-operated goup. ^# P < 0.05, ^## P < 0.01 vs. pMCAO group at the same time points. c Representative images of brain slices stained by TTC in sham-operated group, pMCAO group, bloodletting group. d Quantitative analysis of cerebral infarct area by TTC staining. **P < 0.01 vs. sham-operated group