Fenugreek extract induced vacuoles in Jurkat cells. Overnight incubation of Jurkat cells with 1 mg/mL of fenugreek extract induced distinct vacuoles. (A) The number of vacuoles range from 1 to several per cell. Arrow points to multiple vacuoles in a cell. (B) Arrows point to Jurkat cells possessing two large spherical vacuoles. Pictures represent inverted light microscopic images taken at 400x magnification.