Fig. 6

si-SIRT-1 reduces the APS-induced anti-aging effect in RAECs. (a) Western blotting bands showing SIRT-1, p53 and p21 expression. RAECs were pretransfected with siRNA-NC and then treated with normal medium (Con), 100 µmmol/L H₂O₂ (H₂O₂), or 200 µg/mL APS with H₂O₂ (H₂O₂ + APS) or were transfected with si-SIRT-1 (si-SIRT-1) and then treated with H₂O₂ and APS (HG + APS + si-SIRT-1). (b) Quantification of T-AOC in each group. (c-e) Relative intensities of SIRT-1, p53 and p21 protein bands are shown. (f) Representative images of SA-β-Gal-stained cells in each group. Magnification: 200×. Scale bar = 100 μm. (g) β-Gal-positive cells were quantified in each group. Values are the means ± S.D. (n = 6). ** p < 0.01 vs. control group; #p < 0.05, ## p < 0.01 vs. H₂O₂ group; ^p < 0.05, ^^p < 0.01 vs. H₂O₂ + APS group