Fig. 1

Tan IIA inhibited H1688 and H466 cell proliferation. H1688, H446, BEAS-2B and HBE cells treated with Tan IIA (0, 1, 2, 4 or 6 µM) for 24, 48 or 72 h. (A-D) H1688, H446, BEAS-2B and HBE cell viability determined by MTT assay (n = 3). (E) Colony formation assays of H1688 and H446 cell growth; scale bars: 10 mm, (n = 3). (F) Flow cytometric analysis of apoptotic cells double-stained with Annexin V-FITC and PI. Compared with the control group (n = 3), ns > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001