Fig. 4
Effects of AM on cell proliferation in HeLa cells. a Western blot analysis displaying the reduced expression of cyclin E and CDK1/2 and the complete inhibition of expression of total-AKT, phospho-AKT, and phospho-mTOR upon treatment with IC50 (2.6 µg/mL) and IC90 (6.25 µg/mL) concentrations of AM for 12 h. The representative images are cropped from larger membranes. Original images of blots are shown in Fig. S7. b Relative band intensities of cyclin E and CDK1/2 in cells treated with AM to those of non-treated cells support the decrease in their expression upon AM treatment. Data are represented as mean ± SD and statistically analyzed by one-way ANOVA. Statistically significant differences were observed between the treated groups and the non-treated group; *, p < 0.05; #, p < 0.01; n = 3