Fig. 10

TGT induces apoptosis in osteosarcoma cells. (A-B) Flow cytometry detected that TGT induces apoptosis in 143B and SAOS2 cells. (C) The cytotoxic effect of TGT on osteosarcoma cells can be reversed by co-administration with the apoptosis inhibitor Z-VAD-FMK. (D) Co-administration of TGT with the autophagy inhibitor 3-MA only reverses the cytotoxic effect on osteosarcoma 143B cells at low concentrations. (E) Changes in MMP in osteosarcoma cells treated with TGT observed under fluorescence microscopy. Data are presented as mean ± SD (n = 5). *P < 0.1, **P < 0.01, and ****P < 0.0001 versus the Control group