Table 2 The primer sequences for real-time PCR
Target gene (s) | Primer sequence |
|---|---|
Rock1 | Forward (F) primer:5’-AGAAAGAGGACTTGATTTCCCCGTGC-3’ |
Reverse (R) primer: 5’- ACGGACAAAGCCAGATGGTGGG-3’ | |
MLCK | Forward (F) primer:5’-AGAAGTCAAGGAGGTAAAGAATGATGT-3’ |
Reverse (R) primer: 5’- CGGGTCGCTTTTCATTGC-3 | |
MYPT1 | Forward (F) primer:5’-AAGCGCTCCGTCGTCGTCCT-3’ |
Reverse (R) primer: 5’- TCCCCGGGAGTAGGCAGAGGT-3’ | |
NF-κB | Forward (F) primer: 5’-CTGGTGGACACATACAGGAAGAC-3’ |
Reverse (R) primer: 5’-ATAGGCACTGTCTTCTTTCACCTC-3’ | |
IL-1β | Forward (F) primer: 5’-GCTGCTACTCATTCACTGGCAA-3’ |
Reverse (R) primer: 5’-TGCTGCTGGTGATTCTCTTGTA-3’ | |
Beta actin | Forward (F) primer:5’-ACCGTGAAAAGATGACCCAG-3’ |
Reverse (R) primer: 5’- TCTCAGCTGTGGTGGTGAAG-3’ |