Fig. 8

Liver section from (A) normal group showed negative expression for IL-6 immunostaining, (B) EE-induced intrahepatic cholestasis groupshowed marked positive expression of IL-6 as a brownish cytoplasmic stain in hepatocytes (black arrow), in bile duct lining (red arrow), (C) UDCA-treated group showed mild positive expression of IL-6 as a brownish cytoplasmic stain in hepatocytes (black arrow), in bile duct lining (red arrow), (D) SM-treated group showed moderate positive expression of IL-6 as a brownish cytoplasmic stain in hepatocytes (black arrow), in bile duct lining (red arrow), (E) nano-SM treated group showed mild positive expression of IL-6 as a brownish cytoplasmic stain in hepatocytes (black arrow), (F) % of IL-6 immunostaining (DAB, IL 6, x400). The results are presented as means ± SE. ^{a, b, c, d} Significant difference from normal, EE, EE + UDCA, and EE + SM groups at p < 0.05, respectively. EE: 17α-ethinylestradiol; UDCA: Ursodeoxycholic acid; SM: Silybum marianum L.; IL-6: interleukine-6