Fig. 2

ROS production, loss of mitochondrial membrane potential (ΔΨmt), and caspases activity in nontumor (HEK-293T) and colon (HT-29) and prostate (PC-3) cancer cell lines after being exposed to three different concentrations (C1 = 5 µg*mL^− 1, C2 = 10 µg*mL^− 1, C3 = 25 µg*mL^− 1) of EpSH and ErSA extracts, respectively. (a) and (b) Mean percentage of cells with ROS production. (c) and (d) Mean percentage of cells with ΔΨmt. (e) and (f) Mean percentage of cells with active caspases. For the ROS production, 1 µM daunorubicine (DRN) as a positive control (C+), for the mitochondrial membrane potential, 1 µM Carbonylcyanide-p-trifluoromethoxyphenylhydrazine (FCCP) as a positive control (C+), while 1 µM daunorubicin (DRN) was used as a positive control (C+) for caspase activation. The solvent control was 0.1% ethanol (CS) in all cases. ^A−E Different letters and ^*#+X symbols correspond to significant differences among treatments per cell line (p < 0.05)