Fig. 2

Cytopathic effect (CPE) of two different concentrations (100 and 500 µg/mL) of Tox-S from C. difficile RT001 clinical strain and two different concentrations (50 and 75 µg/mL) of decocted (DEC) and ethanol (ETOH) extracts on Vero cells. (A) Microscopic cell morphology of Vero cells after treatment with Tox-S, DEC and ETOH extracts for 4, 8, 24 h at 37 °C in comparison with untreated cells. Light microscopy (200X magnification), Scale bar = 100 μm. (B) Percentage of Vero cell rounding induced by Tox-S, DEC and ETOH extracts for 4, 8, 24 h at 37 °C in comparison with untreated cells. Data shown are means ± SD of three independent experiments