Fig. 3

BCA abrogates RANKL-induced osteoclastogenesis in a dose-dependent manner. A Chemical structure of BCA. B The effects of the different concentrations of BCA (0, 1.25, 2.5, 5, 10 and 20µM) on BMMs for 48 h were measured by CCK-8 assay. C The number of osteoclasts per well (96-well plate) was quantitatively analyzed. TRAcP-positive multinucleated cells (> 3 nuclei) were identified as osteoclasts. D Representative images of TRAcP staining after treatment with BCA at increasing concentrations (Scale bars = 200 μm). E Representative confocal images of osteoclasts stained for F-actin belts (red) and nuclei (blue), and hydroxyapatite resorption images; the images include osteoclasts treated with RANKL and 0, 5 and 10µM BCA (Scale bars = 200 μm). F Quantitative analysis of osteoclasts in each well (96-well plate). G Quantitative analysis of resorbed proportion per osteoclast. (Data were presented as mean ± SD. Significant differences between the Model and other groups were shown as * (p-value < 0.05), *** (p-value < 0.001))