Table 1 Primer sequences used for RT-qPCR
Gene | Forward (5’ to 3’) | Reverse (5’ to 3’) |
|---|---|---|
Mus Collagen I | GACGCCATCAAGGTCTACTG | ACGGGAATCCATCGGTCA |
Mus α-SMA | ACTGGGACGACATGGAAAAG | GTTCAGTGGTGCCTCTGTCA |
Mus SIRT3 | CACGTTTACAAACATGAACC | CATGCTAGATTGCCCTAGT |
Mus IL-6 | GAGGATACCACTCCCAACAGACC | AAGTGCATCATCGTTGTTCATACA |
Mus IL-1β | GACCTTCCAGGATGAGGACA | AGCTCATATGGGTCCGACAG |
Mus TNF-α | CGTCGTAGCAAACCACCAAG | TTGAAGAGAACCTGGGAGTAGACA |
Mus GAPDH | ACCCAGAAGACTGTGGATGG | ACACATTGGGGGTAGGAACA |
Hum Collagen I | TGACCTCAAGATGTGCCACT | ACCAGACATGCCTCTTGTCC |
Hum α-SMA | CCCGGGACTAAGACGGGAAT | CCATCACCCCCTGATGTCTG |
Hum SIRT3 | GAGGTTCTTGCTGCATGTGGTTG | AGTTTCCCGCTGCACAAGGTC |
Hum IL-6 | CTGCAGCCACTGGTTCTGT | CCAGAGCTGTGCAGATGAGT |
Hum IL-1β | CGATGCACCTGTACGATCAC | TCTTTCAACACGCAGGACAG |
Hum TNF-α | TGCACTTTGGAGTGATCGGC | ACTCGGGGTTCGAGAAGATG |
Hum GAPDH | TGATGACATCAAGAAGGTGGTGAAG | TCCTTGGAGGCCATGTGGGCCAT |