Fig. 5

Deletion of SIRT3 eliminates the anti-inflammatory effects of Diosmin. A, B Western blotting for the transfection efficiency. C-E The effect of SIRT3 SiRNA on the secretion of IL-1β, IL-6, and TNF-α regulated by Diosmin in the supernatant of the culture medium of HK-2 cells. F-H The effect of 3-TYP (SIRT3 selective inhibitor) on the secretion of IL-1β, IL-6, and TNF-α regulated by Diosmin in the supernatant of the culture medium of HK-2 cells. ^**p < 0.01, and ^***p < 0.001 versus the control (DMSO) group; ^&p < 0.05, ^&&p < 0.01, and ^&&&p < 0.001 versus the TGF-β1 alone group; ^#p < 0.05, ^##p < 0.01 and ^###p < 0.001 versus the Dios + TGF-β1 group; ^$$$p < 0.001 versus the SIRT3 siRNA alone group; ns = no significanc