Fig. 4

Diosmin increases SIRT3 expression in vivo and in vitro. A Representative images of SIRT3 IHC staining. B The enzyme activity of SIRT3 in left kidney of different groups of mice. C The mRNA expression levels of SIRT3 in the left kidney and (D) HK-2. (E) Representative western blot analysis and (F) quantitative analysis in the HK-2. ^**p < 0.01, and ***p < 0.001 versus the Sham or the control (DMSO) group; ^&p < 0.05, ^&&p < 0.01, and ^&&&p < 0.001 versus UUO or TGF-β1 group; ^###p < 0.001; ns = no significance; Ctrl = control; Dios = 75uM; Dios-1 = Diosmin 50 mg/kg; Dios-2 = Diosmin 100 mg/kg