Fig. 5
From: Indigofera suffruticosa aerial parts extract induce G2/M arrest and ATR/CHK1 pathway in Jurkat cells
ISAE increased γ-H2A.X expression in Jurkat cells. A Jurkat cells were treated with indicated concentrations (73, 146, 292 µg/mL) of ISAE for 24 h. The expression of γ-H2A.X (phosphorylation of histone H2A.X at serine 139 (phospho-H2A.X (S139)) was revealed by immunofluorescence staining. Nuclei were stained with Hoechst 33258. Bar: 35 μm. B Quantitative results of panel (A). The graph presents the percentage of positive phospho-H2A.X (S139) staining cells of six fields for each slide. * indicates significant difference from control group (***p < 0.001, one-way ANOVA). ISAE significantly increased the number of γ-H2A.X foci positive cells