Fig. 3
From: Indigofera suffruticosa aerial parts extract induce G2/M arrest and ATR/CHK1 pathway in Jurkat cells
ISAE activated ATR-CHK1-Wee1-CDC25C-CDK1 axis in Jurkat cells. Jurkat cells were treated with ISAE at indicated concentrations (36, 73, 146 µg/mL) for indicated times (12, 24, 48, 72 h). Cells were harvested for western blot analysis. A The expressions of Cyclin-dependent kinases 1 (CDK1), phospho-CDK1 (p-CDK1 Y15), p-CDK1 (T161), and Cyclin B1. B The expressions of p-Wee1 (S642), Wee1, and p-CDC25 (S216). C The expressions of p-CHK1 (S345), p-CHK2 (T68), CHK1, and CHK2. D The expressions of ataxia-telangiectasia-mutated protein kinases (ATM), p-ATM (S1981), ATM- and Rad3-related protein kinases (ATR), p-ATR (T1989), and p-ATR (S428). ISAE increased the phosphorylation of CDK1 at tyrosine 15 (Y15), the phosphorylation of Wee1 at serine 642 (S642), the inhibitory phosphorylation of CDC25C at serine 216 (S216), the phosphorylation of CHK1 at serine 345 (S345), and phosphorylation of ATR at both threonine 1989 (T1989) and serine 428 (S428). The original images of each blot can be found in the Supplementary materials