Fig. 2

ISAE induced G2/M arrest and apoptosis in Jurkat cells. A Jurkat cells were exposed to indicated concentrations (36, 73, 146, 292 µg/mL) of ISAE for 24 h, then fixed for propidium iodide (PI) staining. Cell cycle profiles were recorded by flow cytometry. The Dotplot for cell gating of this experiment were presented in Supplementary Fig. S4B-C Quantitative results of G2/M phase (B) and sub-G1 phase (C) of panel (A). D Jurkat cells were exposed to indicated concentrations (36, 73, 146, 292 µg/mL) of ISAE for 24 h, then harvested for annexin V staining and flow cytometry analysis. Representative histogram was showed in Supplementary Fig. S1. E Jurkat cells were treated with 146 µg/ml ISAE for indicated times (12, 24, 48, 72 h). Cell cycle profile were determined as described in (A). F Quantitative results of G2/M phase of panel (E). Data are presented as mean ± SD from three independent experiments. * indicates significant difference from control group (**p < 0.01, ***p < 0.001, one-way ANOVA)