Fig. 1
From: Indigofera suffruticosa aerial parts extract induce G2/M arrest and ATR/CHK1 pathway in Jurkat cells
The I. suffruticosa aerial parts extract (ISAE) caused cytotoxic effects in Jurkat cells. A-C Jurkat (A), HL-60 (B), and KG-1 cells (C) were treated with indicated concentrations (36, 73, 146, 292 µg/mL) of ISAE for 72 h. Resazurin reagent was used to determine cell viability. Distilled water was used as solvent control for 100% viability. D-E The levels of live-cell protease activity (D) and dead-cell protease activity (E) in Jurkat cells after 24 h of ISAE treatment were measured by the Multitox – Glo multiplex cytotoxicity assay. F Human normal dermal fibroblasts (Ccd-966sk) were treated with the indicated concentrations (36, 73, 146, 292 µg/mL) of ISAE for 72 h. Cell viability was measured as described in (A). Data are presented as the mean ± SD from three independent experiments. * indicates significant difference from control group (***p < 0.001, one-way ANOVA). ISAE inhibited the viability of Jurkat cells but had no obvious cytotoxic effects on HL-60, KG-1, and Ccd-966sk cells