Fig. 9

MBE and delphinidins ameliorated cell death induced by inhibiting complex I of the mitochondrial respiratory chain in 661W cells. (A) Representative images of Hoechst 33342 (blue) and PI (magenta) staining in 661W cells incubated with 10 µM rotenone and/or with MBE, D3S5G, and D3G5G for 24 h. Arrowheads indicate PI-positive nuclei. Bar = 100 μm. (B) Quantification of the cell death ratio. Data are shown as the mean ± S.E.M (n = 6). **P < 0.01, Student’s t-test. vs. control (Cont); ^†P < 0.05, ^††P < 0.01, ^†††P < 0.001, Dunnett’s test vs. vehicle (Veh)