Fig. 6

Blue light-induced morphological changes of mitochondria preceded apoptosis. (A) Representative images of mitochondria (MitoTracker, red), externalized phosphatidylserine (annexin V, green), and nuclei (DAPI, blue) in 661W cells exposed to blue light for the indicated time. Arrowheads indicate apoptotic cells. (B) Representative images of mitochondria (Tomm20, red), ER (calreticulin, green), and nuclei (DAPI, blue) in 661W cells exposed to blue light for 8 h with 10 µg/mL MBE, 10 µM D3S5G, or 10 µM D3G5G. Enlarged views of the boxed regions are shown in the upper right corners. Bars = 50 μm (A), 20 μm (B)