Fig. 5

MBE, delphinidins, and Mdivi-1 prevented mitochondrial fragmentation induced by blue light exposure. (A) Representative images of 661W cells stained with MitoTracker (red) and DAPI (blue) following blue light exposure for the indicated times with or without 10 µg/mL MBE, 10 µM D3S5G, 10 µM D3G5G, or 30 µM mitochondrial division inhibitor 1 (Mdivi-1). Enlarged views of the indicated area are shown at the bottom. Bar = 20 μm. (B–G) Quantitative results for the number of mitochondria per cell (B) and average area (C), form factor (D), aspect ratio (E), branch number (F), and branch length (G) of each mitochondrion. A total of 36 cells from three independent experiments were analyzed. The average of each experiment is shown by a solid line. **P < 0.05, **P < 0.01, ***P < 0.001, Tukey’s test vs. control (Cont); ^††P < 0.01, ^†††P < 0.001, Dunnett’s test vs. vehicle (Veh)