Fig. 2

Delphinidins inhibited the blue light-induced cell damage in 661W cells. (A) Chemical structures of D3S5G and D3G5G. (B) Representative images of Hoechst 33342 (blue) and PI (magenta) staining in 661W cells incubated with or without 10 µM D3S5G or 10 µM D3G5G following blue light exposure. Arrowheads indicate PI-positive nuclei. Bar = 100 μm. (C, D) Quantification of the cell death ratio in 661W cells with D3S5G (C) or D3G5G (D). (E, F) Relative levels of ROS in 661W cells with D3S5G (E) or D3G5G (F). MBE was added at 10 µg/mL as a control. Data are shown as the mean ± S.E.M (n = 6) **P < 0.01, ***P < 0.001, Tukey’s test vs. control (Cont); ^†P < 0.05, ^††P < 0.01, ^†††P < 0.001, Dunnett’s test vs. vehicle (Veh); ^##P < 0.01, ^###P < 0.001, Dunnett’s vs. Veh