Fig. 1
MBE and NAC suppressed the elevation of cell death and ROS production in 661W cells exposed to blue light. (A) The diagram depicts the timeline of MBE treatment and blue light exposure. Cells were incubated in the presence of MBE (1, 3, 10 µg/mL) or N-acetyl cysteine (NAC, 1 mM) with or without blue light irradiation (450 lx) for 24 h, followed by cell death assay and ROS measurement. (B) Representative images of Hoechst 33342 (blue) and PI (magenta) staining. Arrowheads indicate PI-positive nuclei. Bar = 100 μm. (C) The cell death ratio is the percentage of PI-positive to Hoechst 33342-positive cells. (D) Relative ROS production determined by fluorescence intensity of CM-H2DCFDA is presented as relative units compared to the control. Bars are shown as the mean ± S.E.M (n = 6). ***P < 0.001, Tukey’s test vs. control (Cont); ††P < 0.01, †††P < 0.001, Dunnett’s test vs. vehicle (Veh); #P < 0.05, ###P < 0.001, Dunnett’s vs. Veh