Table 1 Primers used in real-time PCR.
Gene | Primer sequence | Amplicon Size |
|---|---|---|
miRNA1 | F: 5′-ACACAGAGAGGGCTCCGGCA-3′ R:5′-ACACGACCGTCCACCAACGC-3′ | 342 bp |
miRNA 29b | F: 5′-GCTGGTTTCATATGGTGG-3′ R: 5′-GAACATGTCTGCGTATCTC-3′ | 141 bp |
U6 (internal control for qRT-PCR) | F: ‘5- GCTTCGGCAGCACATATACTAAAAT − 3′; R: 5′- CGCTTCACGAATTTGCGTGTCAT − 3′. | 84 bp |