Fig. 8

Calycosin inhibits IL-1β induced inflammation in vitro. a ADTC5 cells groups and intervention. e Representative images of immunofluorescence staining of Col-2 and Sox-9. b-d Representative western blot bands of COX-2, EGFR and MMP-9 are shown in the CON, MOL, DMSO, and calycosin treatment groups. GAPDH was used as a loading control. The protein levels normalized with GAPDH were shown as fold change relative to the control group. f–h The quantification analysis of the expression levels of COX-2, p-EGFR and MMP-9. i, j Quantitative analysis of the Col-2 and Sox-9 immunofluorescence intensity in each group. Data are presented as the mean ± SD