Fig. 5

NCP was treated with PAM induces apoptosis of SK-N-SH through the cleaving of caspase3 and PARP. A. Western blot analysis of PARP and cleaved caspase 3 showed that SK-N-SH cells were treated with plasma for NT (plasma non-treat), 3 min, and 5 min, and then later for 1 h, 3 h, 6 h, and 24 h. Western blot was performed. GAPDH was used as a loading control for Western blot analysis. B. Quantification of the protein was performed to evaluate the effects of NCP-PAM treatment in SK-N-SH cells. Vertical axis: fold of NT. * indicates p < 0.05, ** indicates p < 0.01