Fig. 5

Effects of ammidin (0, 10, 20 and 40 μM) and PRGL493 concentrations (0, 1, 5 and 10 μM) on cardiomyocytes in the normal and H/R groups. (A-B) Cardiomyocyte viability. (C-D) LDH release. (E-H) ACSL4 protein expression (^*P < 0.05 vs. control; ^#P < 0.05 vs. H/R; n = 3). Proteins were then transferred onto PVDF membranes. After cutting according to the molecular size, the primary antibody was incubated. H/R, hypoxia/reoxygenation; LDH, lactate dehydrogenase; ACSL4, Acyl-CoA synthetase long chain family member 4