Fig. 2
Determination of the beating frequency and cardiomyocyte area at different time points (50–56 h), and effects of Na2S2O4 concentrations (0, 1, 2, 4, 8 mM) on cardiomyocytes after a 1-h treatment. (A) Morphology under light microscopy at 0–72 h. (B) Immunofluorescence staining: Merge: a combination of cTnT-positive cells (green fluorescence) and nuclear DAPI staining (blue fluorescence). (C) Beating frequency during culture for 50–56 h. (D) Diastolic and contractile area during culture for 50–56 h. (E) Viability of cardiomyocytes. (F) Changes in cell morphology (cells that were clearly changing were marked by arrows). (G) Cell beating frequency. (H) Cell diastolic and contractile area. (I) LDH Release rate (*P < 0.05 vs. control; scale bars, 100 μm; n = 3). Na2S2O4, sodium dithionite; DAPI, 4′,6-diamidino-2-phenylindole; LDH, lactate dehydrogenase; cTnT, cardiac troponin T