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Table 1 Qualitative and quantitative phytochemical composition of LMPGE and LMPBE

From: Immunomodulatory effects of epiphytic Loranthus micranthus leaf extracts collected from two host plants: Psidium guajava and Parkia biglobosa

S/N

Phyto-chemicals

Qualitative analysis

Quantitative analysis

Assay

Observation

LMPGE

LMPBE

LMPGE (mg/100 g)

LMPBE (mg/100 g)

p -values

1

Steroid

Cone. H2SO4

Reddish brown colouration

+

+

4.44 + 0.003a

4.43 + 0.005a

0.1971

2

Tannin

Braymer’s test

Greenish brown precipitate

+

+

2.87 + 0.003b

1.97 + 0.003a

0.0163

3

Soluble carbohydrates

Molish’s test

Purple interfacial ring

+

+

0.96 + 0.004 a

1.43 + 0.002 b

< 0.0001

4

Cyanogenic glycoside

 

Brown ring

+

+

0.07 + 0.004b

0.05 + 0.002 a

0.0006

5

Saponins

Frothing’s test

Persistence foaming

+

+

2.03 + 0.004b

1.22 + 0.002a

<  0.0001

6

Reducing sugars

Fehling’s test

Brick red precipitate

+

+

56.52 + 0.003b

43.49 + 0.003 a

<  0.0001

7

Glycoside

Sulphuric acid test

Brick red precipitate

+

+

1.19 + 0.003b

0.99 + 0.002a

< 0.0001

8

Flavonoids

Ethylacetate test

Intense yellow colouration

+

+

5.36 + 0.003b

4.79 + 0.003a

0.0306

9

Alkaloids

Wagner’s test

Reddish-brown ppt

+

+

13.39 + 0.009b

11.35 + 0.002a

< 0.0001

10

Terpenoids

 

Grey coloration

+

+

0.49 + 0.003a

0.48 + 0.001a

0.8593

11

Resin

Acetic anhydride test

No orange-to-yellow coloration

12

Fats and oil

Spot test/ Stain test

No oil stain on the filter paper

  1. The key, +, indicates “the presence or availability of phytochemical” while -, indicate their absence (Qualitative results). The data of the quantitative analysis represent the mean ± SD of triple determination. Values with different superscripts in a row are significantly different, while mean values with the same letter as a superscript are not significantly different at p < 0.05. The results were analyzed with ANOVA, and post hoc multiple comparisons of each mean value were done with the T-test
  2. Quantitative values with different superscripts (a or b) in a row are significantly different, while mean values with the same letter (a) as a superscript are not significantly different at p < 0.05