S/N | Phyto-chemicals | Qualitative analysis | Quantitative analysis | |||||
---|---|---|---|---|---|---|---|---|
Assay | Observation | LMPGE | LMPBE | LMPGE (mg/100 g) | LMPBE (mg/100 g) | p -values | ||
1 | Steroid | Cone. H2SO4 | Reddish brown colouration | + | + | 4.44 + 0.003a | 4.43 + 0.005a | 0.1971 |
2 | Tannin | Braymer’s test | Greenish brown precipitate | + | + | 2.87 + 0.003b | 1.97 + 0.003a | 0.0163 |
3 | Soluble carbohydrates | Molish’s test | Purple interfacial ring | + | + | 0.96 + 0.004 a | 1.43 + 0.002 b | < 0.0001 |
4 | Cyanogenic glycoside | Brown ring | + | + | 0.07 + 0.004b | 0.05 + 0.002 a | 0.0006 | |
5 | Saponins | Frothing’s test | Persistence foaming | + | + | 2.03 + 0.004b | 1.22 + 0.002a | < 0.0001 |
6 | Reducing sugars | Fehling’s test | Brick red precipitate | + | + | 56.52 + 0.003b | 43.49 + 0.003 a | < 0.0001 |
7 | Glycoside | Sulphuric acid test | Brick red precipitate | + | + | 1.19 + 0.003b | 0.99 + 0.002a | < 0.0001 |
8 | Flavonoids | Ethylacetate test | Intense yellow colouration | + | + | 5.36 + 0.003b | 4.79 + 0.003a | 0.0306 |
9 | Alkaloids | Wagner’s test | Reddish-brown ppt | + | + | 13.39 + 0.009b | 11.35 + 0.002a | < 0.0001 |
10 | Terpenoids | Grey coloration | + | + | 0.49 + 0.003a | 0.48 + 0.001a | 0.8593 | |
11 | Resin | Acetic anhydride test | No orange-to-yellow coloration | – | – | – | – | – |
12 | Fats and oil | Spot test/ Stain test | No oil stain on the filter paper | – | – | – | – | – |