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Table 1 The primer sequences for the real-time PCR

From: Artichoke (Cynara scolymus L.) water extract alleviates palmitate-induced insulin resistance in HepG2 hepatocytes via the activation of IRS1/PI3K/AKT/FoxO1 and GSK-3β signaling pathway

Gene

Forward (5' > 3')

Reverse (5' > 3')

IRS1

CCCAGGACCCGCATTCAAA

GGCGGTAGATACCAATCAGGT

PI3K p100α

CCCAGGTGGAATGAATGGCT

AGCACCCTTTCGGCCTTTAA

AKT

AGCGACGTGGCTATTGTGAAG

GCCATCATTCTTGAGGAGGAAGT

GLUT2

GCCTGGTTCCTATGTATATCGGT

GCCACAGATCATAATTGCCCAAG

PEPCK

GGCTGAGAATACTGCCACACT

ACCGTCTTGCTCTCTACTCGT

G6Pase

ACTGGCTCAACCTCGTCTTTA

CGGAAGTGTTGCTGTAGTAGTCA

ATF6α

AGCAGCACCCAAGACTCAAAC

GCATAAGCGTTGGTACTGTCTGA

GRP78

CATCACGCCGTCCTATGTCG

CGTCAAAGACCGTGTTCTCG

CHOP

TTCTCTGGCTTGGCTGACTG

TCCTCCTCTTCCTCCTGAGC

GAPDH

TCCAAAATCAAGTGGGGCGA

TGATGACCCTTTTGGCTCCC

  1. IRS1 Insulin receptor substrate 1, PI3K p100α Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha, Akt Serine/threonine kinase 1, GLUT2 Glucose Transporter 2, PEPCK Phosphoenolpyruvate carboxykinase 2, G6Pase Glucose-6-phosphatase catalytic subunit 1, ATF6α Activating transcription factor 6 alpha, GRP78 G protein-coupled receptor 78, CHOP C/EBP homologous protein, GAPDH Glyceraldehyde-3-phosphate dehydrogenase
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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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