Fig. 6

AWE suppressed the expression of ER stress sensors in IR HepG2 cells. HepG2 cells were exposed to 0.2 mM PA in the absence or presence of 0.25 mg/mL and 0.5 mg/mL AWE for 24 h and then incubated with 100 nM insulin for 30 min. A Representative Western blot images of ATF6, GRP78, CHOP, and GAPDH. B-D Quantitative analysis of ATF6 (B), GRP78 (C), CHOP (D). GAPDH is used as a loading control for protein normalization. E–G Relative mRNA expression of ATF6 (E), GRP78 (F), and CHOP (G) was quantified by qRT-PCR. mRNA levels of target genes were normalized to GAPDH. The data are expressed as mean ± SD. n = (3—6) per group. ^**P < 0.01 vs. Control group. ^##P < 0.01 vs. IR group. The experiment was repeated three times