Fig. 5

AWE enhanced the expression of key molecules involved in glycogenesis in IR HepG2 cells. HepG2 cells were exposed to 0.2 mM PA in the absence or presence of 0.25 mg/mL and 0.5 mg/mL AWE for 24 h and then incubated with 100 nM insulin for 30 min. A Representative Western blot images of GSK3β (Ser9), GSK3β, p-GS (Ser641), GS, and GAPDH. B-E Quantitative analysis of GSK3β (Ser9) (B), GSK3β (C), p-GS (Ser641) (D), GS (E). GAPDH is used as a loading control for protein normalization. The data are expressed as mean ± SD. n = 3 per group. ^**P < 0.01 vs. Control group. ^#P < 0.05, ^##P < 0.01 vs. IR group. The experiment was repeated three times