Fig. 2

AWE improved glucose metabolism in IR HepG2 cells. HepG2 cells were exposed to 0.2 mM PA with or without indicated concentrations (0.1, 0.25, and 0.5 mg/mL) of AWE or 100 µM metformin (Met) for 24 h and then treated with 100 nM insulin for 30 min. A The glucose uptake was detected by 2-NDBG assay. B The fluorescence intensity of these fluorescent images was analyzed using Image J software. C-E Glucose consumption (C), glucose production (D) and the cellular glycogen content (E) were measured. The data are expressed as mean ± SD. n = 6 per group. ^**P < 0.01 vs. Control group. ^#P < 0.05 vs. IR group. The experiment was repeated three times