Fig. 5

Effects of SZYQD water extract on LPS induced IL-6 expression in M-HS cells EGF induced activation of AKT signalling in H292 cells. (A) M-HS cells were exposed to LPS (1 µg/mL) and SZYQD water extract (50 µg/mL and 200 µg/mL) for 24 h. The expression levels of IL-6 were examined by qPCR. (B) Supernatants of M-HS cells were collected and IL-6 secretion levels were measured by ELISA. (C) The expression levels of p-p65 were measured by western blot after LPS (1 µg/mL) and SZYQD extract (50 µg/mL and 200 µg/mL) treatment for 1 h. (D) Quantification of p-p65. (E) H292 cells were exposed to EGF (50 ng/mL) and SZYQD water extract (50 µg/mL and 200 µg/mL) for 24 h. The expression levels of MUC5AC were examined by qPCR. (F) The expression levels of p-AKT and p-EGFR were measured by western blot after EGF (50 ng/mL) and SZYQD water extract (50 µg/mL and 200 µg/mL) treatment for 2 h. (G-H) Quantification of p-EGFR and p-AKT. The data were expressed as mean ± SD (n = 3) of each group. *p < 0.05, **p < 0.01