Fig. 5

PRE inhibited the activation of the NF-kB pathway in vitro. a Representative western blotting images showing TNF-ɑ, IL-6, p-IKB, IKB, p-P65, P65 and TLR4 expression levels. b-d Quantification of TLR4, TNF-α, and IL-6 by ImageJ. e The mean fold change of p-IKB/IKB ratio. f The mean fold change of p-P65/P65 ratio. Data are presented as the mean ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001