Table 1 Oligonucleotide primers for real-time RT-PCR (Human source)
Gene name | Primer | Sequence (5′–3′) | Product size (nt) |
|---|---|---|---|
LXRα | Forward | CTTCAGAACCCACAGAGATCC | 126 |
Reverse | AGCTCAGAACATTGTAGTGGAA | 126 | |
SREBP1c | Forward | AGCAGCAGCAGCAGCAATGG | 105 |
Reverse | CGCCGAGGGAGAGAAGGAAGG | 105 | |
SRB1 | Forward | GGAGATCCCTATCCCCTTCTAT | 123 |
Reverse | CTGAACTCCCTGTACACGTAG | 123 | |
ABCA1 | Forward | TTTTTGCTCAGATTGTCTTGCC | 126 |
Reverse | TGTACTGTTCGTTGTACATCCA | 126 | |
ABCG1 | Forward | CTCCTATGTCAGGTATGGGTTC | 177 |
Reverse | AAAATCCCGAGTACGATGAAGT | 177 | |
ABCG5 | Forward | CAATGTGCTAAAGGGTGCTATC | 101 |
Reverse | GAAACAGATTCACAGCGTTCAG | 101 | |
ABCG8 | Forward | GGTCACGGCGGCAAGATCAAG | 135 |
Reverse | GGTCTCTCGCACAGTCAAGTTGG | 135 | |
CYP7A1 | Forward | GGAAAACCTCCAACGTATCATG | 213 |
Reverse | GGAAAGACTTTGTCGAATTGCT | 213 | |
LDLR | Forward | CTGTAGGGGTCTTTACGTGTTC | 142 |
Reverse | GTTTTCCTCGTCAGATTTGTCC | 142 | |
IDOL | Forward | ACATCAAAGGAGGTGTATGACC | 82 |
Reverse | TCTGGTTGTTTCTTGAAACGAG | 82 |