Table 2 Antibody specificities for IHC and IF.
Target | Supplier | Cat. # | Assay | Dilution |
|---|---|---|---|---|
Primary antibodies | ||||
MUC1 | Ventana | 790–4574 | IHC | rtu |
MUC4 | Ventana | 406 M-16 | IHC | 1:30 |
MUC5AC | ThermoFisher Scientific | MA5-12178 | IHC, AlB-IF | 1:500, 1:100 |
MUC5B | Invitrogen | PA5-82342 | AlB-IF | 1:100 |
PanCK | Ventana | MON5164-1 | IHC | 1:400 |
S100 | Agilent Dako | GA504 | IHC | rtu |
Ki67 | Agilent Dako | GA626 | IF | 1:50 |
cPARP | Cell Signaling | 5625 | IF | 1:50 |
ALOX15 | ThermoFisher Scientific | TA504250 | WB | 1:1000 |
Secondary antibodies | ||||
Goat-anti-mouse IgG Alexa Fluor 546 | ThermoFisher Scientific | A-11,003 | AlB-IF, IF | 1:400 |
Goat-anti-rabbit IgG Alexa Fluor 633 | ThermoFisher Scientific | A-21,070 | AlB-IF, IF | 1:400 |