Fig. 6

Immunohistochemical detection of cleaved caspase-3 protein in the liver. (a-c) Photomicrographs of liver sections of rats from the experimental groups, bar = 50 μm. (a) In control group, showing negative immunoreaction for cleaved caspase-3. (b) In UA group, showing highly positive immunoreaction as represented by brown color specially in the nuclei of hepatocytes. (c) In GA + UA group, showing negative immunoreaction in most of the hepatocytes except few ones still with brown nuclei. (d) Percentage of area of cleaved caspase-3 protein expression in the different experimental groups. Results are expressed as mean ± SEM of 3 rats per group (One-way ANOVA followed by Duncan post-test). # significant difference between UA and the control groups & significant difference between GA + UA and UA groups