Fig. 4

Quantitative evaluation of mRNA expression levels in the brain that changed in microarray analysis. The mRNA expression levels of Trem2 transcript variants 1 and 2 in the hippocampus were measured using real-time reverse transcription polymerase chain reaction. Data were normalized with respect to a housekeeping gene, actin-β, and shown as boxplots (n = 8). Boxplots show minimum, first-quartile, median, third-quartile, and maximum values. Outliers are shown as circles. P-values were determined using one-way analysis of variance (post-hoc test Tukey’s test), * P < 0.05, ** P < 0.01