Fig. 6

Effects of PF on cytoplasmic p65 and nuclear p65 protein levels in LPS-stimulated RAW 264.7 macropages. RAW 264.7 cells were pretreated with PF (0, 3, 6, and 12 μM) for 1 h, followed by the addition of LPS (1 μg/mL) for 24 h. The protein levels of cytoplasmic p65 and nuclear p65 were examined by Western blot analysis. β-actin and Lamin B1 were used as loading controls. Neither LPS nor PF-treated group was selected as control. Data are expressed as mean ± SD from three independent experiments. ## p < 0.01, ### p < 0.001 compared with control group; * p < 0.05, ** p < 0.01, *** p < 0.001 compared with LPS alone-treated group