Fig. 7
Cell viability assay of ethanol extract of L. sinense (LSE) on breast cancer cells. A Graph showing relative cell viability in multiple cell lines treated with L. sinense ethanol extract (LSE) at the indicated concentration for 48 h. Cell-Titer Glo® assay was performed to measure cell viability. Data are mean ± SD; n = 3 samples per group. ns, not significant; *P < 0.05; **P < 0.01 and ***P < 0.001 by the Two-way ANOVA. B IC50 values of L. sinense ethanol extract (LSE) against tested breast cancer cell lines. IC50 values were derived by a dose–response (Variable slope) curve using GraphPad Prism software. Dose–response data points represent the mean value of three independent experiments. C Representative phase contrast microscopy images showing the morphology change of MDA-MB-468 cells treatment with LSE in 2D and 3D cultures. Scale bar: 50 µm. Bar plot showing the relative cell viability change (Cell-Titer Glo® assay) of MDA-MB-468 cells with indicated treatment in 3D culture. Data are mean ± SD. ns, not significant; *P < 0.05; **P < 0.01 by the Student's t-test