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Table 1 Real-time PCR primer sequences

From: A crossover randomized controlled trial examining the effects of black seed (Nigella sativa) supplementation on IL-1β, IL-6 and leptin, and insulin parameters in overweight and obese women

 

Forward

Reverse

IL-1β

TGATGGCTTATTACAGTGGCAAT

AAGCCCTTGCTGTAGTGGTG

IL-6

CCCTGAGAAAGGAGACATG

TGATTTTCACCAGGCAAGTC

Leptin

TCCCCTCTTGACCCATCTC

GGGAACCTTGTTCTGGTCAT

GAPDH

ACAACTTTGGTATCGTGGAAGG

GCCATCACGCCACAGTTTC

  1. Abbreviations: PCR, polymerase chain reaction; IL-1β, interleukin-1 beta; IL-6, interleukin-6; GAPDH, glyceraldehyde-3-phosphate dehydrogenase
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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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