Fig. 5

AO/EB staining. Fluorescence microscopy images of control, 5-fluorouracil (positive control) and RE-treated A549 cells. Apoptosis induction in cells was evaluated by AO/EB staining at 48 h. (A) Control (0 µg/ml); (B) 5-fluorouracil (10 µg/ml); and (C) IC₅₀ value of RE (430 µg/ml). White arrows indicate apoptotic cells. Uniform green color – live cells; green with bright green dots – early apoptotic cells; orange – late apoptotic cells