Table 2 Primer sequences for the target genes
Gene | Rrimer Sequence | |
|---|---|---|
Forward primer | Reverse primer | |
β-actin | 5'-TGTGACGTGGACATCCG-3' | 5'-ATCTTCATTGTGCTGGGTG-3' |
VEGF | 5'-ATCTTCATTGTGCTGGGTG-3' | 5'-TTCACCACTTCGTGATGATTCTG-3' |
MDM2 | 1TrA1TrAAAGTCTGTYGGTGCA-3 | 5'-TGAAGG1TITCTC1TrCCTGAAG-3' |
P53 | 5'-CTTTGAGGTGCGTGTTT-3' | 5'-CAGTGCTCGCTTAGTGC-3' |
Bax | 5'-TGAACCGGCATCTGCACAC-3' | 5'-AACATGTCAGCTGCCACTCG-3' |
Bcl-2 | 5'-TGAACCGGCATCTGCACAC-3' | 5'-TGAACCGGCATCTGCACAC-3' |
Caspase-3 | 5'-CTGGACTGTGGCATTGAGAC-3 | 5'-ACAAAGCGACTGGATGAACC-3' |
HIF-1α | 5'-GCTGGCCCCAGCCGCTGGAG-3' | 5'-GAGTGCAGGGTCAGCACTAC-3' |
EGFR | 5'-ATCTGCCTCACCTCCACCGT-3' | 5'-GCAGGTACTGGGAGCCAATA-3' |